Human PAI1+tPA ELISA Kit (ab192151)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 16 pg/ml
- Range: 39 pg/ml - 2500 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human PAI1+tPA ELISA Kit
See all PAI1 + tPA kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 6.3% Inter-assay Sample n Mean SD CV% Overall 3 14.7% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
16 pg/ml -
Range
39 pg/ml - 2500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 88 82% - 91% Serum 89 80% - 100% Cell culture media 99 92% - 108% Hep Plasma 103 93% - 111% EDTA Plasma 67 66% - 70% Cit plasma 82 80% - 84% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human PAI1+tPA ELISA kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency. This version will be discontinued when inventory is depleted. The new version is available under ab269559.
Human PAI1+tPA ELISA Kit (ab192151) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PAI1+tPA protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PAI1+tPA with 16 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Tissue plasminogen activator (tPA or PLAT) is a serine protease that catalyzes the conversion of plasminogen to plasmin, which is integral to blood clot breakdown. Plasminogen activator inhibitor-1 (PAI-1 or Serpin E1) is a serine protease inhibitor that blocks the function of tPA. PAI-1 and tPA form a complex that is stable and non-covalent. The PAI-1/tPA complex appears to be predictive of risk for myocardial infarction. This assay uses a PAI-1 specific capture antibody and a tPA-specific detector antibody to specifically detect PAI1/tPA complexes in Human biological samples. The reference protein is a non-covalent complex of PAI 1 and tPA.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X PAI1-tPA complex Capture Antibody 1 x 600µl 10X PAI1-tPA Complex Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml PAI1-tPA Complex Human Lyophilized Purified Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Cellular localization
Secreted -
Alternative names
- Alteplase
- Clade E
- Endothelial plasminogen activator inhibitor
see all -
Database links
- Entrez Gene: 5327 Human
- Entrez Gene: 5054 Human
- Omim: 173370 Human
- Omim: 173360 Human
- SwissProt: P05121 Human
- SwissProt: P00750 Human
Images
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Other - Human PAI1+tPA ELISA Kit (ab192151)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of PAI1-TPA complex standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of PAI1-TPA complex in Human serum and plasma (heparin).The concentrations of PAI1-TPA complex were measured in duplicate and interpolated from the PAI1-TPA complex standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PAI1-TPA complex concentration was determined to be 2,040 pg/mL in serum and 2,160 pg/mL in plasma (heparin).
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Interpolated concentrations of PAI1-TPA complex in Human serum from 10 male donors.Serum from 10 apparently healthy male donors was measured in duplicate. The mean PAI1-TPA complex concentration was determined to be 4,126 pg/mL with a range of 2,764- 5,584 pg/mL in male donors.
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Determination of the specificity of the PAI-TPA complex ELISA.Recombinant proteins for PAI1, TPA, and PAI1-TPA complex were tested in PAI1 SimpleStep ELISA, TPA SimpleStep ELISA and PAI1-Complex SimpleStep ELISA to determine specificity of the PAI-TPA complex ELISA. Background-subtracted data values are graphed. The PAI1-TPA Complex assay does not detect free PAI1 or TPA protein.
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Comparison of secreted PAI1-TPA complex protein, PAI1 protein and TPA protein in Human serum and Human plasma (citrate) samples.PAI1-TPA complex, PAI1 protein and TPA protein were measured in 8-fold diluted serum and plasma. PAI1, TPA, and PAI1-TPA complex values were measured and interpolated from their respective Standard Curve diluted in Sample Diluent NS or Sample Diluent 25BP. Mean +/-SD, n=2, are graphed.
