Human PAI1 + tPA ELISA Kit (ab108892)
Key features and details
- Sensitivity: 0.06 ng/ml
- Range: 0.063 ng/ml - 4 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Tissue
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human PAI1 + tPA ELISA Kit
See all PAI1 + tPA kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5.2% Inter-assay Sample n Mean SD CV% Overall 10.2% -
Sample type
Cell culture supernatant, Serum, Plasma, Tissue -
Assay type
Sandwich (quantitative) -
Sensitivity
= 0.06 ng/ml -
Range
0.063 ng/ml - 4 ng/ml -
Recovery
98 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s PAI1 + tPA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PAI1 + tPA concentrations in plasma, serum, tissue extracts and cell culture supernatants.
A PAI1 + tPA specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a PAI1 + tPA specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of PAI1 + tPA captured in plate.
Get results in 90 minutes with Human PAI1+tPA ELISA Kit (ab192151) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 20ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human PAI1 + tPA Antibody 1 x 120µl Chromogen Substrate 1 x 8ml PAI1 + tPA Microplate (12 x 8 well strips) 1 unit PAI1 + tPA Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Cellular localization
Secreted -
Alternative names
- Alteplase
- Clade E
- Endothelial plasminogen activator inhibitor
see all -
Database links
- Entrez Gene: 5327 Human
- Entrez Gene: 5054 Human
- Omim: 173370 Human
- Omim: 173360 Human
- SwissProt: P05121 Human
- SwissProt: P00750 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab108892.
