Human P4HB ELISA Kit (ab277425)
Key features and details
- Sensitivity: 0.22 ng/ml
- Range: 0.205 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human P4HB ELISA Kit
See all P4HB kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.22 ng/ml -
Range
0.205 ng/ml - 50 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 113.2 98% - 138% Plasma 98.54 90% - 117% Cell culture media 115.2 94% - 139% -
Species reactivity
Reacts with: Human -
Product overview
Human P4HB ELISA kit (ab277425) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human P4HB in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human P4HB coated on a 96-well plate. Standards and samples are pipetted into the wells and Human P4HB present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human P4HB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human P4HB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated Anti-Human P4HB Antibody 2 vials Anti-Human P4HB coated Microplate 1 unit Human P4HB standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. -
Sequence similarities
Belongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains. -
Cellular localization
Endoplasmic reticulum lumen. Melanosome. Cell membrane. Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources (Probable). Localizes near CD4-enriched regions on lymphoid cell surfaces. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Alternative names
- Cellular thyroid hormone binding protein
- Cellular thyroid hormone-binding protein
- Collagen prolyl 4 hydroxylase beta
see all -
Database links
- Entrez Gene: 5034 Human
- Omim: 176790 Human
- SwissProt: P07237 Human
- Unigene: 464336 Human
- Unigene: 708779 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.
