Anti-Lactoferrin antibody (ab77548)
Key features and details
- Goat polyclonal to Lactoferrin
- Suitable for: WB, Sandwich ELISA, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Lactoferrin antibody
See all Lactoferrin primary antibodies -
Description
Goat polyclonal to Lactoferrin -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumansELISA Recombinant fragmentWB Human
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Immunogen
Synthetic peptide:
C-ENYKSQQSSDPDP
, corresponding to internal sequence amino acids 432-444 of Human Lactoferrin (NP_002334.2). -
Positive control
- WB: Human Peripheral Blood Mononucleocyte lysate. IHC: Human blood vessel.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab77548 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Lactoferrin antibody (ab77548)Anti-Lactoferrin antibody (ab77548) at 0.05 µg/ml + Human Peripheral Blood Mononucleocyte lysate (in RIPA buffer) at 35 µg
Observed band size: 85 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactoferrin antibody (ab77548)ab77548 (2µg/ml) staining of paraffin embedded Human Blood Vessel shows strong staining of cytoplasm of neutrophils. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. -
Sandwich ELISA - Anti-Lactoferrin antibody (ab77548)Sandwich-type ELISA with increasing amount of recombinant LTF captured by a rabbit antibody. ab77548 reporter concentration: 5 ug/ml -
Western blot - Anti-Lactoferrin antibody (ab77548)
