Human OLFM2 ELISA Kit (ab277477)
Key features and details
- Sensitivity: 0.45 ng/ml
- Range: 0.41 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human OLFM2 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.45 ng/ml -
Range
0.41 ng/ml - 100 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 102.1 79% - 124% Plasma 79.98 69% - 94% Cell culture media 121.4 99% - 141% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human OLFM2 ELISA Kit (ab277477) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human OLFM2 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human OLFM2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human OLFM2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human OLFM2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human OLFM2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated Anti-Human OLFM2 Antibody 2 vials Anti-Human OLFM2 coated Microplate 1 unit Human OLFM2 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Involved in transforming growth factor beta (TGF-beta)-induced smooth muscle differentiation. TGF-beta induces expression and nuclear translocation of OLFM2 where it binds to SRF, causing its dissociation from the transcriptional repressor HEY2/HERP1 and facilitating binding of SRF to target genes (PubMed:25298399). Plays a role in AMPAR complex organization. -
Tissue specificity
Expressed in aortic smooth muscle (at protein level). -
Sequence similarities
Contains 1 olfactomedin-like domain. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted. Cell junction, synapse. Membrane. Nucleus. Cytoplasm. Nuclear localization is induced by TGF-beta. - Information by UniProt
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Alternative names
- Neuronal olfactomedin related ER localized protein 2
- NOE2
- NOE2_HUMAN
see all -
Database links
- Entrez Gene: 93145 Human
- SwissProt: O95897 Human
- Unigene: 169743 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.