Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Otx1 + Otx2 antibody [EPR3347] - BSA and Azide free (ab247578)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3347] to Otx1 + Otx2 - BSA and Azide free
  • Suitable for: IHC-P, WB, ChIP, IP
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-Otx1 + Otx2 antibody [EPR3347] - BSA and Azide free
    See all Otx1 + Otx2 primary antibodies

  • Description

    Rabbit monoclonal [EPR3347] to Otx1 + Otx2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ChIP, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human, Recombinant fragment

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247578 is the carrier-free version of ab92515 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247578 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Otx1 + Otx2 antibody [EPR3347] - BSA and Azide free (ab247578)
    Western blot - Anti-Otx1 + Otx2 antibody [EPR3347] - BSA and Azide free (ab247578)
    Anti-Otx1 + Otx2 antibody [EPR3347] - ChIP Grade (ab92515) at 1/2500 dilution (purified) + Y79 cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

    Predicted band size: 32 kDa
    Observed band size: 32 kDa



    This data was developed using ab92515, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 + Otx2 antibody [EPR3347] - BSA and Azide free (ab247578)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 + Otx2 antibody [EPR3347] - BSA and Azide free (ab247578)

    This data was developed using ab92515, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human retina tissue labelling Otx1 + Otx2 with purified ab92515 at a dilution 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 (ab93684). ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Counterstained with hematoxylin. Nuclear staining on inner nuclear layer and outer nuclear layer of the human retina.
  • ChIP - Anti-Otx1 + Otx2 antibody [EPR3347] - BSA and Azide free (ab247578)
    ChIP - Anti-Otx1 + Otx2 antibody [EPR3347] - BSA and Azide free (ab247578)

    This data was developed using ab92515, the same antibody clone in a different buffer formulation.

    Chromatin was prepared from mouse stem cell D3 according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab92515 (blue), and 20µl of Anti rabbit IgG sepharose beads. 5μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
  • Immunoprecipitation - Anti-Otx1 + Otx2 antibody [EPR3347] - BSA and Azide free (ab247578)
    Immunoprecipitation - Anti-Otx1 + Otx2 antibody [EPR3347] - BSA and Azide free (ab247578)

    ab92515&

    44; the same antibody clone in a different buffer formulation.

    >Otx1 + Otx2 was immunoprecipitated from Y79 (Human retinoblastoma retinoblastoma) whole cell lysate with ab92515 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab92515 at 1/500 dilution. Secondary antibody details (ab131366), was used as secondary antibody at 1/1000 dilution.

    locking and dilution buffer: 5% NFDM/TBST.



    Rabbit monoclonal IgG (ab172730) instead of ab92515 + Y79 whole cell lysate at 10 µg

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
  • Immunoprecipitation - Anti-Otx1 + Otx2 antibody [EPR3347] - BSA and Azide free (ab247578)
    Immunoprecipitation - Anti-Otx1 + Otx2 antibody [EPR3347] - BSA and Azide free (ab247578)

    ab92515&

    44; the same antibody clone in a different buffer formulation.

    >Otx1 + Otx2 was immunoprecipitated from Y79 (Human retinoblastoma retinoblastoma) whole cell lysate with ab92515 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab92515 at 1/500 dilution. Secondary antibody details (ab131366), was used as secondary antibody at 1/1000 dilution.

    locking and dilution buffer: 5% NFDM/TBST.



    Lane 2 : Anti-Otx1 + Otx2 antibody [EPR3347] - ChIP Grade (ab92515) at 1/500 dilution (purified)
    Lane 3 : purified

    All lanes : Y79 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
  • Immunoprecipitation - Anti-Otx1 + Otx2 antibody [EPR3347] - BSA and Azide free (ab247578)
    Immunoprecipitation - Anti-Otx1 + Otx2 antibody [EPR3347] - BSA and Azide free (ab247578)

    ab92515&

    44; the same antibody clone in a different buffer formulation.

    >Otx1 + Otx2 was immunoprecipitated from Y79 (Human retinoblastoma retinoblastoma) whole cell lysate with ab92515 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab92515 at 1/500 dilution. Secondary antibody details (ab131366), was used as secondary antibody at 1/1000 dilution.

    locking and dilution buffer: 5% NFDM/TBST.



    Lanes 1-2 : Anti-Otx1 + Otx2 antibody [EPR3347] - ChIP Grade (ab92515) at 1/500 dilution (purified)
    Lane 3 : purified

    Lane 1 : Y79 (Human retinoblastoma retinoblastoma) whole cell lysate
    Lanes 2-3 : Y79 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
  • Western blot - Anti-Otx1 + Otx2 antibody [EPR3347] - BSA and Azide free (ab247578)
    Western blot - Anti-Otx1 + Otx2 antibody [EPR3347] - BSA and Azide free (ab247578)
    Anti-Otx1 + Otx2 antibody [EPR3347] - ChIP Grade (ab92515) at 1 µg/ml (unpurified) + OTX1 Recombinant Protein at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under non-reducing conditions.

    Predicted band size: 32 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab92515, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 + Otx2 antibody [EPR3347] - BSA and Azide free (ab247578)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 + Otx2 antibody [EPR3347] - BSA and Azide free (ab247578)

    This data was developed using ab92515, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human retinoblastoma tissue labelling Otx1 + Otx2 with purified ab92515 at a dilution 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 (ab93684). ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Counterstained with hematoxylin. Nuclear staining on tumor cells of human retinoblastoma.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 + Otx2 antibody [EPR3347] - BSA and Azide free (ab247578)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 + Otx2 antibody [EPR3347] - BSA and Azide free (ab247578)

    This data was developed using ab92515, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse retina tissue labelling Otx1 + Otx2 with purified ab92515 at a dilution 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 (ab93684). ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Counterstained with hematoxylin. Nuclear staining on nuclear layer of the mouse retina.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 + Otx2 antibody [EPR3347] - BSA and Azide free (ab247578)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 + Otx2 antibody [EPR3347] - BSA and Azide free (ab247578)
    This data was developed using ab92515, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse E14.5 choroid plexus tissue labelling Otx1 + Otx2 with purified ab92515 at a dilution 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 (ab93684). ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Counterstained with hematoxylin. Nuclear staining on mouse E14.5 choroid plexus.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 + Otx2 antibody [EPR3347] - BSA and Azide free (ab247578)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 + Otx2 antibody [EPR3347] - BSA and Azide free (ab247578)
    This data was developed using ab92515, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat E14.5 choroid plexus tissue labelling Otx1 + Otx2 with purified ab92515 at a dilution 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 (ab93684). ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Counterstained with hematoxylin. Nuclear staining on rat E14.5 choroid plexus.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 + Otx2 antibody [EPR3347] - BSA and Azide free (ab247578)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 + Otx2 antibody [EPR3347] - BSA and Azide free (ab247578)
    This data was developed using ab92515, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab92515 at a working dilution of 1/250. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Anti-Otx1 + Otx2 antibody [EPR3347] - BSA and Azide free (ab247578)
    Anti-Otx1 + Otx2 antibody [EPR3347] - BSA and Azide free (ab247578)

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