Human NT-proBNP ELISA Kit (ab263877)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 11.5 pg/ml
- Range: 21.9 pg/ml - 1400 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human NT-proBNP ELISA Kit
See all BNP/ProBNP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.3% Inter-assay Sample n Mean SD CV% Serum 3 5.6% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
11.5 pg/ml -
Range
21.9 pg/ml - 1400 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 90 84% - 94% Hep Plasma 89 84% - 93% EDTA Plasma 89 88% - 90% Cit plasma 90 85% - 94% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human NT-proBNP ELISA Kit (ab263877) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NT-proBNP protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human NT-proBNP with 11.5 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
NT-proBNP, or Brain Natriuretic Peptide, is a hormone expressed by cardiomyocytes by the gene NPPB. NT-proBNP is synthesized as a 134- amino acid preprohormone (prepro-BNP), subsequent removal of a 25 amino acid signal peptide forms pro-BNP, which is stored intracellularly. Pro-BNP is secreted as an inactive prohormone and cleavage by furin or corin forms NT-pro-BNP and the active 32- amino acid BNP-32 peptide. BNP may function as a paracrine antifibrotic factor in the heart, as well as have a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. The antibody pair in this product detect NT-pro-BNP.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human NT-proBNP Capture Antibody 1 x 600µl 10X Human NT-proBNP Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human NT-proBNP Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. -
Tissue specificity
Brain and also in atria, but at much lower levels than ANP. -
Sequence similarities
Belongs to the natriuretic peptide family. -
Post-translational
modificationsThe brain natriuretic peptide 32 form is cleaved at Pro-104 by the prolyl endopeptidase FAP (seprase) activity (in vitro). -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- ANFB_HUMAN
- BNP
- BNP(1-32)
see all -
Database links
- Entrez Gene: 4879 Human
- Omim: 600295 Human
- SwissProt: P16860 Human
- Unigene: 219140 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human NT-proBNP standard curve in Sample Diluent 50BS.The NT-proBNP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native NT-proBNP in human congestive heart failure serum samples.The concentrations of NT-proBNP were measured in duplicates, interpolated from the NT-proBNP standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 7.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean NT-proBNP concentration was determined to be 16,839 pg/mL in serum.
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Serum from eight individual healthy human female donors was measured in duplicate.Two were positive for Human BNP. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean NT-proBNP concentration was determined to be 159.7 pg/mL with a range of 76.1 – 243.3 pg/mL.
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Sandwich ELISA - Human NT-proBNP ELISA Kit (ab263877)To learn more about the advantages of recombinant antibodies see here.
