Human NLRP3 ELISA Kit (ab274401)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.63 ng/ml
- Range: 4.38 ng/ml - 280 ng/ml
- Sample type: Cell Lysate, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human NLRP3 ELISA Kit
See all NLRP3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 2.5% Inter-assay Sample n Mean SD CV% Extract 3 5.1% -
Sample type
Tissue Extracts, Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
1.63 ng/ml -
Range
4.38 ng/ml - 280 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Tissue Extracts 112 110% - 113% Cell Lysate 112 111% - 112% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human NLRP3 ELISA kit (ab274401) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human NLRP3 protein in cell and tissue extract. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human NLRP3 with 1.63 ng/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
NACHT, LRR and PYD domains-containing protein 3 (NLRP3), encoded by the NLRP3 gene, is known to have a vital role in the innate immunity and inflammation. NLRP3 is highly expressed in macrophages and as a component of the inflammasomes in which it functions to detect products of damaged cells and subsequently activates itself to trigger an immune response. Mutations in the NLRP3 has been shown in studies to be associated with a wide spectrum of dominantly inherited autoimmune diseases. The standard protein provided is an N-terminal fragment of human NLRP3 that has been mass calibrated to full-length recombinant NLRP3.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human NLRP3 Capture Antibody 1 x 600µl 10X Human NLRP3 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml Human NLRP3 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. -
Tissue specificity
Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder. -
Involvement in disease
Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) [MIM:120100]; also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold.
Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) [MIM:191900]; also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs.
Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) [MIM:607115]; also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation. -
Sequence similarities
Belongs to the NLRP family.
Contains 1 DAPIN domain.
Contains 9 LRR (leucine-rich) repeats.
Contains 1 NACHT domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- AGTAVPRL
- AII/AVP
- Angiotensin/vasopressin receptor AII/AVP like
see all -
Database links
- Entrez Gene: 114548 Human
- Omim: 606416 Human
- SwissProt: Q96P20 Human
- Unigene: 159483 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human NLRP3 standard curve in 1X Cell Extraction Buffer PTR.NLRP3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native NLRP3 in human THP-1 and liver samples based on a 62.5 µg/mL and 500 µg/mL extract load, respectively.The concentrations of NLRP3 were measured in duplicate and interpolated from the NLRP3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean NLRP3 concentration was determined to be 8.7 ng/mL in THP-1 cell extract and undetectable in Liver Extract.
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Sandwich ELISA - Human NLRP3 ELISA Kit (ab274401)To learn more about the advantages of recombinant antibodies see here.
