Human Lysozyme ELISA Kit
(ab267798)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 7.31 pg/ml
- Range: 31.25 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Milk, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
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Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 1.7% Inter-assay Sample n Mean SD CV% Serum 3 2.2% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
7.31 pg/ml -
Range
31.25 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 111 107% - 116% Serum 103 99% - 107% Hep Plasma 110 107% - 115% EDTA Plasma 105 107% - 112% Cit plasma 115 113% - 117% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Lysozyme ELISA Kit (ab267798) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Lysozyme protein in edta plasma, hep plasma, milk, saliva, serum, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Lysozyme with 7.31 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Lysozyme is an integral part of the innate immune system where it serves to hydrolyze the1,4-beta linkages of N-acetylmuramic acid and N-acetyl-D-glucosamine. This hydrolysis helps weaken the cell membrane of gram-positive bacteria. It is found in high abundance in secretory fluids, i.e tears, saliva, milk.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Lysozyme Capture Antibody 1 x 600µl 10X Human Lysozyme Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human Lysozyme Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. -
Involvement in disease
Amyloidosis 8 -
Sequence similarities
Belongs to the glycosyl hydrolase 22 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- 1
- 1 4 beta N acetylmuramidase C
- 4-beta-N-acetylmuramidase C
see all -
Database links
- Entrez Gene: 4069 Human
- Omim: 153450 Human
- SwissProt: P61626 Human
- Unigene: 524579 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Lysozyme standard curve in Sample Diluent.The Lysozyme standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Lysozyme in human serum, and plasma samples.The concentrations of Lysozyme were measured in duplicates, interpolated from the Lysozyme standard curves and corrected for sample dilution. Undiluted samples are as follows: 1:2,000 serum, 1:2,000 plasma (citrate), 1:2,000 plasma (heparin) and 1:2,000 plasma (edta). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lysozyme concentration was determined to be 2608.00 ng/mL in serum, 2993.07 ng/mL in plasma (citrate) and 2814.88 ng/mL in plasma (heparin) and 2811.86 ng/mL in plasma (edta).
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Interpolated concentrations of native Lysozyme in human urine, and CSF samples.The concentrations of Lysozyme were measured in duplicates, interpolated from the Lysozyme standard curves and corrected for sample dilution. Undiluted samples are as follows: 1:20 urine, 1;100 CSF. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lysozyme concentration was determined to be 23.33 ng/mL in urine, 78.43 ng/mL in CSF.
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Interpolated concentrations of native Lysozyme in human milk, and saliva samples.The concentrations of Lysozyme were measured in duplicates, interpolated from the Lysozyme standard curves and corrected for sample dilution. Undiluted samples are as follows: 1:100.000 milk, and 1:2,000,000 saliva. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lysozyme concentration was determined to be 150.03 mg/mL in milk, 2,696.20 mg/mL in saliva.
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Serum from ten individual healthy human female donors were measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lysozyme concentration was determined to be 3,932.39 ng/mL with a range of 1,520.16 –6,208.68 ng/mL.
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Sandwich ELISA - Human Lysozyme ELISA Kit (ab267798)To learn more about the advantages of recombinant antibodies see here.
