Human NFKB1 (NFkB p105 / p50) knockout HeLa cell lysate (ab257003)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab260093 - Human NFKB1 knockout HeLa cell lysate 1 x 100µg ab255552 - Human wild-type HeLa cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10
Images
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Western blot - Human NFKB1 (NFkB p105 / p50) knockout HeLa cell lysate (ab257003)Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: NFKB1 knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab32360 observed at 105 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab32360 Anti-NFkB p105 / p50 antibody [E381] was shown to specifically react with NFkB p105 / p50 in wild-type HeLa cells in western blot. The band observed in the knockout cell line ab264823 (knockout cell lysate ab257003) lane below 105kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and NFkB p105 / p50 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32360 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human NFKB1 knockout HeLa cell lysate (ab257003)Allele-1: 1 bp insertion in exon 7; Allele-2: Insertion of the selection cassette in exon 7
