Human Neuropilin-1 Antibody Pair - BSA and Azide free (ab244048)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
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Product name
Human Neuropilin-1 Antibody Pair - BSA and Azide free
See all Neuropilin 1 kits -
Assay type
ELISA set -
Range
37.5 pg/ml - 4800 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
The Antibody Pair can be used to quantify Human Neuropilin-1. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab227901), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human Neprilysin Capture Antibody (unconjugated) 1 x 100µg Human Neprilysin Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis.
The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity. -
Tissue specificity
The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules. -
Sequence similarities
Belongs to the neuropilin family.
Contains 2 CUB domains.
Contains 2 F5/8 type C domains.
Contains 1 MAM domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- A5 protein
- BDCA4
- BLOOD DENDRITIC CELL ANTIGEN 4
see all -
Database links
- Entrez Gene: 8829 Human
- Omim: 602069 Human
- SwissProt: O14786 Human
- Unigene: 131704 Human
- Unigene: 653996 Human
Images
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Sandwich ELISA - Human Neuropilin-1 Antibody Pair - BSA and Azide free (ab244048)To learn more about the advantages of recombinant antibodies see here.