Human Neurogranin ELISA Kit (Trunc P75) (NRGN) (ab277084)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 12.9 pg/ml
- Range: 25 pg/ml - 400 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human Neurogranin ELISA Kit (Trunc P75) (NRGN)
See all Neurogranin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 3.6% Inter-assay Sample n Mean SD CV% Extract 3 6.5% -
Sample type
Serum, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
12.9 pg/ml -
Range
25 pg/ml - 400 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 91 90% - 91% Tissue Extracts 88 82% - 91% Hep Plasma 104 98% - 111% EDTA Plasma 80 76% - 84% Cit plasma 85 84% - 86% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Neurogranin (Trunc P75) ELISA kit (ab277084) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Neurogranin protein in human serum, plasma - citrate, plasma - EDTA, plasma - heparin, and tissue extract. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Neurogranin with 12.9 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
Neurogranin (Trunc P75), encoded by the NRGN gene, is a postsynaptic protein kinase that is highly expressed in the brain and regulates the availability of calmodulin in which neurogranin binds to calmodulin in the absence of calcium. Trunc P75 has found to be up regulated in CSF and downregulated in the brain of Alzhemer’s Disease patients and is a biomarker for Alzheimer’s Disease. ab277084 has been developed to specifically recognize the C Terminus of Neurogranin (Trunc p75).
-
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Neurogranin (Trunc P75) (NRGN) Capture Antibody 1 x 600µl 10X Human Neurogranin (Trunc P75) (NRGN) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human Neurogranin (Trunc P75) (NRGN) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
Acts as a "third messenger" substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium. -
Tissue specificity
In the cerebral cortex, found in the cell bodies of neurons in layers II-VI, and in apical and basal dendrites of pyramidal neurons. Is not found in the dendrites in patients with Alzheimer disease. -
Sequence similarities
Belongs to the neurogranin family.
Contains 1 collagen-like domain.
Contains 1 IQ domain. - Information by UniProt
-
Alternative names
- Protein kinase C substrate RC3
- Calmodulin binding protein
- Hng
see all -
Database links
- Entrez Gene: 4900 Human
- Omim: 602350 Human
- SwissProt: Q92686 Human
- Unigene: 524116 Human
Images
-
SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of Human Neurogranin standard curve in Sample Diluent NS+1X Enhancer.The Neurogranin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
-
Example of pan-hmr Neurogranin standard curve in 1X Cell Extraction Buffer PTR.The Neurogranin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native Neurogranin in human brain extract based on a 40 µg/mL extract load.The concentrations of Neurogranin were measured in duplicate and interpolated from the Neurogranin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neurogranin concentration was determined to be 277.3 pg/mL in Brain tissue extract.
-
Sandwich ELISA - Human Neurogranin Trunc P75To learn more about the advantages of recombinant antibodies see here.
