Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category

Human MTHFD1L knockout HEK-293T cell lysate (ab258976)

Price and availability

318 288 ₸

Availability

Order now and get it on Friday March 05, 2021

Human MTHFD1L knockout HEK-293T cell lysate (ab258976)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

You may also be interested in

Product image
Mouse FAS ELISA Kit (ab213862)
Product image
Recombinant Human CDC42 protein (ab87713)
Product image
Anti-CD55 antibody [EPR22362-255] (ab243231)
Product image
Anti-CDCP1 antibody - BSA and Azide free (Detector) (ab259686)

Overview

  • Product name

    Human MTHFD1L knockout HEK-293T cell lysate
  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    HEK293T
  • Organism

    Human
  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 8 bp deletion in exon2 and Insertion of the selection cassette in exon2.
  • Passage number

  • Knockout validation

    Sanger Sequencing
  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab261404 - Human MTHFD1L knockout HEK293T cell lysate 1 x 100µg
    ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg
  • Cell type

    epithelial
  • STR Analysis

    Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12

Target

  • Function

    May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2.
  • Tissue specificity

    Detected in most tissues, highest expression found in placenta, thymus and brain. Low expression is found in liver and skeletal muscle. Up-regulated in colon adenocarcinoma.
  • Pathway

    One-carbon metabolism; tetrahydrofolate interconversion.
  • Sequence similarities

    In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
    In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.
  • Domain

    This monofunctional enzyme consists of two major domains: an N-terminal inactive methylene-THF dehydrogenase and cyclohydrolase domain and an active larger formyl-THF synthetase C-terminal domain.
  • Cellular localization

    Mitochondrion.
  • Target information above from: UniProt accession Q6UB35 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • 10-formyl-THF synthetase
    • C1TM_HUMAN
    • Formyltetrahydrofolate synthetase
    • formyltetrahydrofolate synthetase domain containing 1
    • FTHFSDC1
    • methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
    • mitochondrial
    • Monofunctional C1-tetrahydrofolate synthase
    • MTC1THFS
    • MTHFD1L
    • RP1-292B18.2
    see all

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab261404 - Human MTHFD1L knockout HEK293T cell lysate 1 x 100µg
    ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg
  • Cell type

    epithelial
  • STR Analysis

    Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12

Images

  • Sanger Sequencing - Human MTHFD1L knockout HEK293T cell lysate (ab258976)
    Sanger Sequencing - Human MTHFD1L knockout HEK293T cell lysate (ab258976)

    Allele-1: 8 bp deletion in exon2

     

  • Sanger Sequencing - Human MTHFD1L knockout HEK293T cell lysate (ab258976)
    Sanger Sequencing - Human MTHFD1L knockout HEK293T cell lysate (ab258976)

    Allele-2: 1 bp insertion in exon2

     

  • Sanger Sequencing - Human MTHFD1L knockout HEK293T cell lysate (ab258976)
    Sanger Sequencing - Human MTHFD1L knockout HEK293T cell lysate (ab258976)

    Allele-3: Insertion of the selection cassette in exon2

     

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

  •  
  • Product image

    Anti-Hydrogen Potassium ATPase Beta/ATP4B antibody [EPR8819(2)] - C-terminal (ab186749)

  •  
  • Product image

    Anti-PP2A alpha + beta antibody [EPR11787(B)] - BSA and Azide free (ab249449)

  •  
  • Product image

    SEAP Reporter Gene Assay Kit (Luminescence) (ab133077)

  •  
  • Product image

    Human PDGFRB knockout SHSY-5Y cell line (ab273749)

  •  
  • Product image

    Recombinant human BCMA protein (Active) (ab269983)

  •  
  • Product image

    Recombinant Human Synaptotagmin VII protein (ab160324)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.