Biotin conjugation kit (with inbuilt quantification) (ab272214) is a unique antibody/protein conjugation system with inbuilt biotin quantification, which obviates the need for a separate determination of incorporated biotin using the HABA assay.

The conjugation procedure is extremely simple: the antibody to be labeled is used to reconstitute a freeze-dried activator compound, and the resulting mixture is added to the biotin chromophore ligand vial. After a fixed time, the conjugate is desalted to remove excess ligand and the absorbance of the conjugate is read at A350 and A280. These absorbance values allow you to determine the yield of conjugate, its concentration, and the average number of biotins per antibody.

Benefits

No need for separate quantification of biotin molecules incorporated

Simple conjugation procedure

Amine-based chemistry – flexibility in choice of antibody

Stable conjugates due to covalent bonding

General notes:

This product is manufactured by Expedeon, an Abcam company, and was previously called Biotin Q conjugation kit (with inbuilt quantification). Expedeon product codes 1600-0010 and 1600-0015 are the same as the current sizes: 3 x 100 μg and 1 x 1 mg respectively.