Biotin conjugation kit (with inbuilt quantification) (ab272214)
Overview
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Product name
Biotin conjugation kit (with inbuilt quantification) -
Product overview
Biotin conjugation kit (with inbuilt quantification) (ab272214) is a unique antibody/protein conjugation system with inbuilt biotin quantification, which obviates the need for a separate determination of incorporated biotin using the HABA assay.
The conjugation procedure is extremely simple: the antibody to be labeled is used to reconstitute a freeze-dried activator compound, and the resulting mixture is added to the biotin chromophore ligand vial. After a fixed time, the conjugate is desalted to remove excess ligand and the absorbance of the conjugate is read at A350 and A280. These absorbance values allow you to determine the yield of conjugate, its concentration, and the average number of biotins per antibody.
Benefits
No need for separate quantification of biotin molecules incorporated
Simple conjugation procedure
Amine-based chemistry – flexibility in choice of antibody
Stable conjugates due to covalent bonding
General notes:
This product is manufactured by Expedeon, an Abcam company, and was previously called Biotin Q conjugation kit (with inbuilt quantification). Expedeon product codes 1600-0010 and 1600-0015 are the same as the current sizes: 3 x 100 μg and 1 x 1 mg respectively.
Properties
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Storage instructions
Please refer to protocols. -
Components 3 x 100 µg 1 x 1 mg ab273936 - Activator 3 x 100µg 1 x 1mg ab273935 - Biotin ligand 3 x 100µg 1 x 1mg ab273931 - Column equilibration buffer 1 x 50ml 1 x 50ml ab273934 - DMSO 1 x 200µl 1 x 200µl ab273937 - NAP-5 Column 3 units 0 unit ab273940 - PD-10 Column 0 unit 1 unit ab273932 - Quencher reagent 1 x 200µl 1 x 200µl ab273933 - Reaction Buffer 1 x 200µl 1 x 200µl
Images
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Protocol steps for the Biotin conjugation kit (with inbuilt quantification)
The antibody to be labelled is used to reconstitute a freeze-dried activator compound, and the resulting mixture is added to the biotin Q chromophore ligand vial.
