Human MMP3 ELISA Kit (ab269371)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 4.4 pg/ml
- Range: 25 pg/ml - 1600 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human MMP3 ELISA Kit
See all MMP3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 2.8% Inter-assay Sample n Mean SD CV% Serum 4 4.7% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
4.4 pg/ml -
Range
25 pg/ml - 1600 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 114 111% - 116% Serum 110 107% - 115% Hep Plasma 110 107% - 111% EDTA Plasma 112 104% - 118% Cit plasma 110 101% - 120% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human MMP3 ELISA Kit (ab269371) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MMP3 protein in cell culture supernatant, edta plasma, hep plasma, serum, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MMP3 with 4.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Matrix metalloprotease-3 (MMP3), also known as Stromelysin-1, is a 54 kD secreted protease involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. MMP3 functions by degrading fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. This also activates pro-collagenase to further help in the remodeling of the extracellular matrix environment.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human MMP3 Capture Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human MMP3 Detector Antibody (Lyophilized) 1 vial Human MMP3 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- CHDS6
- Matrix metalloproteinase 3
- Matrix metalloproteinase 3 preproprotein
see all -
Database links
- Entrez Gene: 4314 Human
- Omim: 185250 Human
- SwissProt: P08254 Human
- Unigene: 375129 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human MMP3 standard curve comparisonStandard Curve comparison between human MMP3 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Human MMP3 standard curve comparisonStandard Curve comparison between the original human MMP3 SimpleStep ELISA kit (ab189572) and current human MMP3 SimpleStep ELISA kit (ab269371).
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Example of human MMP3 standard curve in Sample Diluent NS.The MMP3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native MMP3 in human serum, plasma (EDTA), plasma (citrate), and plasma (heparin) samples.The concentrations of MMP3 were measured in duplicates, interpolated from the MMP3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 6.25%, plasma (EDTA) 17.5%, plasma (citrate) 12.5%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP3 concentration was determined to be 15.3 ng/mL in serum, 7.8 ng/mL in plasma (EDTA), 11.0 ng/mL in plasma (citrate) and 10.4 ng/mL in plasma (heparin).
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Interpolated concentrations of native MMP3 in human MG-63 cell supernatant samples.The concentrations of MMP3 were measured in duplicates, interpolated from the MMP3 standard curves and corrected for sample dilution. Undiluted samples are as follows: MG-63 cell supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP3 concentration was determined to be 1.2 ng/mL in MG-63 cell supernatant.
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Serum from eight individual healthy MMP3 gender donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP3 concentration was determined to be 9.53 ng/mL with a range of 3.6 – 15.5 ng/mL.
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Sandwich ELISA - Human MMP3 ELISA Kit (ab269371)To learn more about the advantages of recombinant antibodies see here.
