Human ADAMTS4 ELISA Kit (ab213753)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 625 pg/ml - 40000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human ADAMTS4 coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti- Human ADAMTS4 antibody 1 x 130µl Lyophilized recombinant Human ADAMTS4 standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml -
Research areas
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Function
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-
-Ala-393' site. -
Tissue specificity
Expressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles. -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 1 TSP type-1 domain. -
Domain
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- A disintegrin and metalloproteinase with thrombospondin motifs 4
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 4
- A disintegrin like and metalloprotease with thrombospondin type 1 motif 4
see all -
Database links
- Entrez Gene: 9507 Human
- Omim: 603876 Human
- SwissProt: O75173 Human
- Unigene: 211604 Human
Images
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Human ADAMTS4 ELISA Kit (ab213753) Standard Curve.
Human ADAMTS4 ELISA Kit (ab213753) Standard Curve.