Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human MCP2 ELISA Kit (ab223856)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 1.1 pg/ml
  • Range: 1.25 pg/ml - 160 pg/ml
  • Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human MCP2 ELISA Kit
    See all MCP2 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Cell Extract 3 3.2%
    Inter-assay
    Sample n Mean SD CV%
    Cell Extract 3 10.9%

  • Sample type

    Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    1.1 pg/ml

  • Range

    1.25 pg/ml - 160 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 132 123% - 146%
    Cell culture extracts 99 84% - 117%
    Hep Plasma 113 112% - 117%
    EDTA Plasma 94 85% - 100%
    Cit plasma 126 115% - 138%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human MCP2 ELISA Kit (ab223856) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MCP2 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MCP2 with 1.1 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Monocyte chemoattractant protein 2 (MCP2), known also as C-C motif chemokine 8 (CCL8) is chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. Chemokines and their receptors, in conjunction with adhesion molecules and their ligands, provide cues to enable tissue-specific homing of T cells in the steady state and during inflammation. The processed form MCP2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and MCP2. MCP2 protein can bind heparin. 

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    10X Human MCP2 Capture Antibody 1 x 600µl
    10X Human MCP2 Detector Antibody 1 x 600µl
    Human MCP2 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 75BS 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8.

  • Tissue specificity

    Highest expression found in the small intestine and peripheral blood cells. Intermediate levels seen in the heart, placenta, lung, skeletal muscle, thymus, colon, ovary, spinal cord and pancreas. Low levels seen in the brain, liver, spleen and prostate.

  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.

  • Post-translational
    modifications

    N-terminal processed form MCP-2(6-76) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • Ccl8
    • CCL8_HUMAN
    • HC14
    • MCP-2
    • MCP-2(6-76)
    • Monocyte chemoattractant protein 2
    • Monocyte chemotactic protein 2
    • SCYA10
    • SCYA8
    • Small-inducible cytokine A8
    see all

  • Database links

    Images

    • Other - Human MCP2 ELISA Kit (ab223856)
      Other - Human MCP2 ELISA Kit (ab223856)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of Human MCP2 standard curve in Sample Diluent 75BS.
      Example of Human MCP2 standard curve in Sample Diluent 75BS.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native MCP2 in Human serum and plasma samples.
      Interpolated concentrations of native MCP2 in Human serum and plasma samples.

      The concentrations of MCP2 were measured in duplicates, interpolated from the MCP2 standard curves. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, plasma (heparin) 100% and plasma (EDTA) 100%. The interpolated values are plotted (mean +/- SD, n=2). The mean MCP2 concentration was determined to be 19 pg/mL in neat serum, 16 pg/mL in neat plasma (citrate), 18 pg/ml in neat plasma (heparin), and 15 pg/mL in neat plasma (EDTA).

    • Interpolated concentrations of native MCP2 in Human 1.5%-PHA-treated and untreated PBMC (2 days) supernatants.
      Interpolated concentrations of native MCP2 in Human 1.5%-PHA-treated and untreated PBMC (2 days) supernatants.

      The concentrations of MCP2 were measured in duplicates, interpolated from the MCP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC treated supernatant 0.08%, PBMC untreated supernatant 0.08%. The interpolated dilution factor corrected values of treated PBMC supernatants are plotted (mean +/- SD, n=2). The mean MCP2 concentration was determined to be 80,341 pg/mL in neat treated PBMC supernatant and undetectable in untreated supernatants (not shown).

    • Example of Human MCP2 standard curve in Sample Diluent NS.
      Example of Human MCP2 standard curve in Sample Diluent NS.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Example of Human MCP2 standard curve in 1X Cell Extraction Buffer PTR.
      Example of Human MCP2 standard curve in 1X Cell Extraction Buffer PTR.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of spike MCP2 in Human serum and plasma samples diluted in Sample Diluent 75BS.
      Interpolated concentrations of spike MCP2 in Human serum and plasma samples diluted in Sample Diluent 75BS.

      The concentrations of MCP2 were measured in duplicates, interpolated from the MCP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, plasma (heparin) 100% and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

    • Interpolated concentrations of native MCP2 in Human 1.5%, 2-day PHA-treated PBMC extract, based on a 50 µg/mL extract load.
      Interpolated concentrations of native MCP2 in Human 1.5%, 2-day PHA-treated PBMC extract, based on a 50 µg/mL extract load.

      The concentrations of MCP2 were measured in duplicate and interpolated from the MCP2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP2 concentration was determined to be 37 pg/ml in treated PBMC extract and undetectable in untreated PBMC extract (not shown).

    • Serum from ten individual healthy Human male donors was diluted four-fold and measured in duplicate.
      Serum from ten individual healthy Human male donors was diluted four-fold and measured in duplicate.

      Interpolated, dilution factor-corrected values are plotted (mean +/- SD, n=2). The mean MCP2 concentration was determined to be 21 pg/mL with a range of 12-32 pg/mL in neat serum.

    • Sandwich ELISA - Human MCP2 ELISA Kit (ab223856)
      Sandwich ELISA - Human MCP2 ELISA Kit (ab223856)
      To learn more about the advantages of recombinant antibodies see here.

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