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Signal Transduction Protein Phosphorylation Ser / Thr Kinases MAPK Pathway

Human MAP2K4 (MEK4/MKK4) knockout HeLa cell lysate (ab263802)

Price and availability

318 288 ₸

Availability

Order now and get it on Tuesday March 09, 2021

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Overview

  • Product name

    Human MAP2K4 (MEK4/MKK4) knockout HeLa cell lysate
  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    HeLa
  • Organism

    Human
  • Passage number

  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab261646 - Human MAP2K4 knockout HeLa cell lysate 1 x 100µg
    ab255929 - Human wild-type HeLa cell lysate 1 x 100µg
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway
  • Cell type

    epithelial
  • Disease

    Adenocarcinoma
  • Gender

    Female

Target

  • Function

    Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
  • Tissue specificity

    Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
  • Target information above from: UniProt accession P45985 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • c Jun N terminal kinase kinase 1
    • C-JUN N-terminal kinase kinase 1
    • Dual specificity mitogen activated protein kinase kinase 4
    • Dual specificity mitogen-activated protein kinase kinase 4
    • JNK Activated Kinase 1
    • JNK activating kinase 1
    • JNK-activating kinase 1
    • JNKK
    • JNKK1
    • MAP kinase kinase 4
    • Map2k4
    • MAPK ERK kinase 4
    • MAPK/ERK kinase 4
    • MAPKK 4
    • MAPKK4
    • MEK 4
    • MEK4
    • Mitogen activated protein kinase kinase 4
    • MKK 4
    • MKK4
    • MP2K4_HUMAN
    • PRKMK4
    • SAPK ERK kinase 1
    • SAPK/ERK kinase 1
    • SAPKK 1
    • SAPKK1
    • SEK1
    • SERK1
    • SKK1
    • Stress activated protein kinase kinase 1
    see all

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab261646 - Human MAP2K4 knockout HeLa cell lysate 1 x 100µg
    ab255929 - Human wild-type HeLa cell lysate 1 x 100µg
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway
  • Cell type

    epithelial
  • Disease

    Adenocarcinoma
  • Gender

    Female

Images

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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