Human MANF ELISA kit (ab215417)
Key features and details
- Sensitivity: 30 pg/ml
- Range: 0.25 ng/ml - 16 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human MANF ELISA kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Plasma #1 16 6.37ng/ml 4.48% Plasma #2 16 2.13ng/ml 4.77% Serum #1 16 1.02ng/ml 7.84% Serum #2 16 2.64ng/ml 3.71% Inter-assay Sample n Mean SD CV% Plasma #1 4 7.1ng/ml 9.9% Plasma #2 4 2.3ng/ml 6.2% Serum #1 4 1.1ng/ml 3% Serum #2 4 2.5ng/ml 3.9% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
30 pg/ml -
Range
0.25 ng/ml - 16 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Spike 101.38 0.25ng/ml - 4ng/ml -
Assay time
2h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The human MANF ELISA kit (ab215417) is designed for the quantitative determination of human MANF in cell culture supernatant, serum and plasma.
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Notes
Microplate wells are pre-coated with anti-human MANF-specific monoclonal capture antibodies. Samples and standards are pipetted into microwells and human MANF molecules present in the sample are bound by the capture antibodies. After incubation, unbound material is removed by washing the wells. Then, horseradish peroxidase (HRP) conjugated human MANF-specific polyclonal detection antibodies bind to different epitopes of human MANF molecules. After washing, the ready to use HRP substrate (TMB) is added to wells. The intensity of the color produced is directly proportional to the amount of human MANF in the sample. Color development is then stopped by the addition of stop solution. Absorbance is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash concentrate 1 x 50ml anti-Human MANF coated microplate (12 x 8 wells) 1 unit Human MANF enzyme conjugate stock 1 x 80µl Human MANF enzyme conjugate stock diluent 1 x 12ml Human MANF sample diluent 1 x 25ml Human MANF standard A (0 ng/mL) 1 x 1ml Human MANF standard B (0.25 ng/mL) 1 x 1ml Human MANF standard C (0.5 ng/mL) 1 x 1ml Human MANF standard D (1 ng/mL) 1 x 1ml Human MANF standard E (2 ng/mL) 1 x 1ml Human MANF standard F (4 ng/mL) 1 x 1ml Human MANF standard G (8 ng/mL) 1 x 1ml Human MANF standard H (16 ng/mL) 1 x 1ml Stop solution 1 x 12ml TMB Substrate solution 1 x 12ml -
Research areas
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Function
Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity). Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. -
Sequence similarities
Belongs to the ARMET family. -
Domain
The N-terminal region may be responsible for neurotrophic activity while the C-terminal region may play a role in the ER stress response. -
Post-translational
modificationsMay contain sialic acid residues. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Arginine rich mutated in early stage tumors
- Arginine rich protein
- Arginine-rich protein
see all -
Database links
- Entrez Gene: 7873 Human
- Omim: 601916 Human
- SwissProt: P55145 Human
- Unigene: 436446 Human
Images
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Human MANF ELISA kit ab215417 Standard Curve.
Cubic spline fit curve on a log-log scale.