Human Luteinizing hormone ELISA Kit (LH) (ab178658)
Key features and details
- Sensitivity: 0.22 mIU/ml
- Range: 0.5 mU/ml - 200 mU/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Luteinizing hormone ELISA Kit (LH) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 20 Inter-assay Sample n Mean SD CV% Serum 15 -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.22 mIU/ml -
Range
0.5 mU/ml - 200 mU/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Luteinizing hormone (LH) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of LH in serum.
A 96-well plate has been precoated with Streptavidin. Samples, standards and the LH-HRP and Bitoin conjugate are added to the wells. Biotinylated monoclonal and enzyme labeled antibodies are added and the reactants mixed: these antibodies have high affinity and specificity and are directed against distinct and different epitopes of LH. Reaction between the various LH antibodies and native LH occurs in the microwells without competition or steric hindrance forming a soluble sandwich complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of LH in the sample and the intensity is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 50X Washing Solution 1 x 20ml Cover Foil 1 unit Luteinizing Hormone Coated Microplate (12 x 8 wells) 1 unit Luteinizing Hormone Control 1 x 1ml Luteinizing Hormone HRP and Biotin Conjugate 1 x 12ml Luteinizing Hormone Standard 0 - 0 mU/mL 1 x 1ml Luteinizing Hormone Standard 1 - 5 mU/mL 1 x 1ml Luteinizing Hormone Standard 2 - 25 mU/mL 1 x 1ml Luteinizing Hormone Standard 3 - 50 mU/mL 1 x 1ml Luteinizing Hormone Standard 4 - 100 mU/mL 1 x 1ml Luteinizing Hormone Standard 5 - 200 mU/mL 1 x 1ml Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml -
Research areas
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Function
Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. -
Tissue specificity
Pituitary gland. -
Involvement in disease
Defects in LHB are a cause of hypogonadism LHB-related (HGON-LHB) [MIM:152780]. Hypogonadism is characterized by infertility and pseudohermaphroditism. -
Sequence similarities
Belongs to the glycoprotein hormones subunit beta family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CGB4
- CHORIONIC GONADOTROPIN, BETA POLYPEPTIDE 4
- hLHB
see all -
Database links
- Entrez Gene: 3972 Human
- Omim: 152780 Human
- SwissProt: P01229 Human
- Unigene: 154704 Human
Images
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Typical Standard Curve
Representative standard curve using ab178658