Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human LGR5 knockout HeLa cell pellet (ab279150)

Overview

  • Product name

    Human LGR5 knockout HeLa cell pellet
    See all LGR5 kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HeLa

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3 and 8 bp deletion in exon3.

  • Passage number

  • Knockout validation

    Sanger Sequencing

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Properties

Target

  • Function

    Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicule. Target gene of Wnt signaling.

  • Tissue specificity

    Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver.

  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
    Contains 16 LRR (leucine-rich) repeats.
    Contains 1 LRRNT domain.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Alternative names

    • FEX
    • G protein coupled receptor 49
    • G protein coupled receptor 67
    • g protein-coupled receptor fex
    • G-protein coupled receptor 49
    • G-protein coupled receptor 67
    • G-protein coupled receptor HG38
    • GPCR GPR49
    • GPR 49
    • GPR 67
    • GPR49
    • GPR67
    • GRP 49
    • GRP49
    • HG 38
    • HG38
    • Leucine rich repeat containing G protein coupled receptor 5
    • Leucine-rich repeat-containing G-protein coupled receptor 5
    • LGR 5
    • LGR5
    • LGR5_HUMAN
    • MGC117008
    • Orphan G protein coupled receptor HG38
    see all

Properties

Images

  • Sanger Sequencing - Human LGR5 knockout HeLa cell pellet (ab279150)
    Sanger Sequencing - Human LGR5 knockout HeLa cell pellet (ab279150)

    Allele-1: 8 bp deletion in exon3

     

  • Sanger Sequencing - Human LGR5 knockout HeLa cell pellet (ab279150)
    Sanger Sequencing - Human LGR5 knockout HeLa cell pellet (ab279150)

    Allele-2: 1 bp insertion in exon3

     

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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