Human C3a ELISA kit (ab279352)
Key features and details
- Sensitivity: 63 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human C3a ELISA kit
See all C3 / C3a kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
63 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 96.46 80% - 105% Plasma 97.3 86% - 105% Cell culture media 94.88 85% - 100% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human C3a ELISA kit (ab279352) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human C3a in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for human C3a coated on a 96-well plate. Standards and samples are pipetted into the wells and C3a present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human C3a antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of C3a bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. -
Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 kit Anti-human C3a coated microplate 1 unit 20X Wash Buffer Concentrate 1 x 25ml Human C3a Standard Protein (Lyophilized) 2 vials Biotinylated anti-human C3a antibody 2 vials 200X HRP-Streptavidin Concentrate 1 x 200µl TMB One-Step Substrate Reagent 1 x 12ml Stop Solution 1 x 8ml 5X Assay Diluent 1 x 15ml -
Research areas
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Function
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
C3-beta-c: Acts as a chemoattractant for neutrophils in chronic inflammation.
Acylation stimulating protein: adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2 (PubMed:8376604, PubMed:2909530, PubMed:9059512, PubMed:10432298, PubMed:15833747, PubMed:16333141, PubMed:19615750). -
Tissue specificity
Plasma. The acylation stimulating protein (ASP) is expressed in adipocytes and released into the plasma during both the fasting and postprandial periods. -
Involvement in disease
Complement component 3 deficiency
Macular degeneration, age-related, 9
Hemolytic uremic syndrome atypical 5
Increased levels of C3 and its cleavage product ASP, are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage. -
Sequence similarities
Contains 1 anaphylatoxin-like domain.
Contains 1 NTR domain. -
Post-translational
modificationsC3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g. C3a is further processed by carboxypeptidases to release the C-terminal arginine residue generating the acylation stimulating protein (ASP). Levels of ASP are increased in adipocytes in the postprandial period and by insulin and dietary chylomicrons.
Phosphorylated by FAM20C in the extracellular medium. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Acylation stimulating protein
- AHUS5
- ARMD9
see all -
Database links
- Entrez Gene: 718 Human
- Omim: 120700 Human
- SwissProt: P01024 Human
- Unigene: 529053 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.