Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human LGALS1 (Galectin 1) knockout HEK293T cell pellet (ab278915)

Overview

  • Product name

    Human LGALS1 (Galectin 1) knockout HEK293T cell pellet
    See all Galectin 1 kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HEK293T

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, Homozygous: 171 bp deletion in exon 2.

  • Passage number

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

Target

  • Function

    May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.

  • Tissue specificity

    Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.

  • Sequence similarities

    Contains 1 galectin domain.

  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • Alternative names

    • 14 kDa laminin-binding protein
    • 14 kDa lectin
    • Beta galactoside binding lectin
    • Beta galactoside binding lectin L 14 I
    • beta galactoside binding protein
    • Beta-galactoside-binding lectin L-14-I
    • DKFZp686E23103
    • Gal 1
    • Gal-1
    • GAL1
    • Galaptin
    • Galbp
    • Galectin
    • Galectin-1
    • Galectin1
    • GBP
    • HBL
    • HLBP14
    • HPL
    • L 14.5
    • L-14.5
    • L14
    • Lactose binding lectin 1
    • Lactose-binding lectin 1
    • Lect14
    • Lectin galactoside binding soluble 1
    • Lectin galactoside-binding soluble 1
    • LEG1_HUMAN
    • LGALS 1
    • LGALS1
    • Lgals1 lectin galactose binding soluble 1
    • MAPK activating protein MP12
    • Putative MAPK activating protein MP12
    • Putative MAPK-activating protein PM12
    • S Lac lectin 1
    • S-Lac lectin 1
    see all

Properties

Images

  • Western blot - Human LGALS1 (Galectin 1) knockout HEK293T cell pellet (ab278915)
    Western blot - Human LGALS1 (Galectin 1) knockout HEK293T cell pellet (ab278915)
    Lane 1: Wild-type HEK-293T cell lysate (20µg)

    Lane 2: LGALS1 knockout HEK-293T cell lysate (20µg)
    Lanes 1- 2: Merged signal (red and green). Green - ab138513 observed at 15 kDa. Red - loading control ab8245 observed at 37 kDa.
    ab138513 Anti-Galectin 1 antibody [EPR3206(2)] was shown to specifically react with Galectin 1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266850 (knockout cell lysate ab256974) was used. Wild-type and Galectin 1 knockout samples were subjected to SDS-PAGE. ab138513 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
  • Sanger Sequencing - Human LGALS1 (Galectin 1) knockout HEK293T cell pellet (ab278915)
    Sanger Sequencing - Human LGALS1 (Galectin 1) knockout HEK293T cell pellet (ab278915)
    Homozygous: 171 bp deletion in exon 2

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up