Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14528(B)] to Peroxiredoxin 5
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Peroxiredoxin 5 antibody [EPR14528(B)]
    See all Peroxiredoxin 5 primary antibodies

  • Description

    Rabbit monoclonal [EPR14528(B)] to Peroxiredoxin 5

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Peroxiredoxin 5 aa 50-150. The exact sequence is proprietary.
    Database link: P30044

  • Positive control

    • HepG2, HeLa, Jurkat and A549 cell lysates; HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR14528(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)
    Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: PRDX5 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HepG2 whole cell lysate (20 µg)
    Lane 4: A549 whole cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab180123 observed at 17 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab180123 was shown to specifically react with PRDX5 when PRDX5 knockout samples were used. Wild-type and PRDX5 knockout samples were subjected to SDS-PAGE. Ab180123 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 10000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)
    Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)
    All lanes : Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123) at 1/20000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa
    Additional bands at: 17 kDa. We are unsure as to the identity of these extra bands.

  • Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)
    Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Peroxiredoxin 5 with ab180123 at 1/250 dilution, followed by Goat anti rabbit IgG (Dylight 555) at 1/250 dilution. Counterstained with DAPI (blue).

  • Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)
    Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)

    Western Blot analysis of HeLa cell lysate immunoprecipitated with ab180123 at 1/60 dilution, using Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.

  • Flow Cytometry - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)
    Flow Cytometry - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Peroxiredoxin 5 with ab180123 at 1/10 dilution (red) compared to a rabbit IgG control (green), followed by Goat anti rabbit IgG (FITC) at 1/75 dilution.

  • Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)
    Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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