Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human Lactate Dehydrogenase B / LDH-B ELISA Kit (ab183367)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 8 pg/ml
  • Range: 0.312 ng/ml - 20 ng/ml
  • Sample type: Cell culture supernatant, Cell Lysate, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Lactate Dehydrogenase B / LDH-B ELISA Kit
    See all Lactate Dehydrogenase B/LDH-B kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    HeLa extract 5 1.9%
    Inter-assay
    Sample n Mean SD CV%
    HeLa extract 3 2.7%

  • Sample type

    Cell culture supernatant, Serum, Tissue Extracts, Cell Lysate, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    8 pg/ml

  • Range

    0.312 ng/ml - 20 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 105 101% - 108%
    Cell culture media 108 108% - 109%
    Hep Plasma 104 102% - 105%
    EDTA Plasma 110 107% - 115%
    Cit plasma 110 107% - 114%
    Extraction Buffer 98 97% - 100%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human Lactate Dehydrogenase B ELISA kit (ab183367) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LDHB protein in human serum, plasma, cell culture supernatant, cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate human LDHB with 8 pg/mL sensitivity.

    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

            -Single-wash protocol reduces assay time to 90 minutes or less
            -High sensitivity, specificity and reproducibility from superior antibodies
            -Fully validated in biological samples
            -96-wells plate breakable into 12 x 8 wells strips

    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.


    Sensitivity:
    Samples diluted in Sample Diluent NS - 8 pg/mL
    Samples diluted in 1X Cell Extraction Buffer PTR - 212 pg/mL

  • Notes

    Functional lactate dehydrogenase are homo or hetero tetramers composed of M and H protein subunits encoded by the LDH-A and LDH-B genes, respectively. The major isozymes of skeletal muscle and liver has four muscle (M) subunits; while the main isozymes for heart muscle contains four (H) subunits. The other variants contain both types of subunits. LDHB converts pyruvate to lactate under low oxygen conditions, while in the LDH-A converts lactate into pyruvate. Elevated levels of LDH indicate tissue breakdown in hemolysis. LDH can be used as a marker of myocardial infarction, peaking at days 3 and lasting through day 10. Other disorders indicated by elevated LDH include cancer, meningitis, encephalitis, acute pancreatitis, and HIV. Defects in LDHB are a cause of hereditary LDH-B deficiency; however LDH-B deficiency is usually asymptomatic.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate

Properties

Images

  • Other - Human Lactate Dehydrogenase B / LDH-B ELISA Kit (ab183367)
    Other - Human Lactate Dehydrogenase B / LDH-B ELISA Kit (ab183367)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of LDHB standard curve diluted in 1X Cell Extraction PTR
    Example of LDHB standard curve diluted in 1X Cell Extraction PTR

    Background-subtracted data values (mean +/- SD) are graphed.

  • Example of LDHB standard curve diluted in NS
    Example of LDHB standard curve diluted in NS

    Background-subtracted data values (mean +/- SD) are graphed.

  • Titration of HeLa and HepG2 extracts within the working range of the assay
    Titration of HeLa and HepG2 extracts within the working range of the assay

    Raw data in duplicate measurements are plotted. Background is represented as the dotted black line.

  • Titration of Human Heart and Human Liver homogenate extracts within the working range of the assay
    Titration of Human Heart and Human Liver homogenate extracts within the working range of the assay

    Raw data in duplicate measurements are plotted. Background is represented as the dotted black line.

  • Quantitation of LDHB expression in different cell lines
    Quantitation of LDHB expression in different cell lines

    Interpolated values of LDHB are plotted for the indicated cell lines based on an extract load of 20 µg/mL.

  • Interpolated concentrations of LDHB in Human serum and plasma titrations
    Interpolated concentrations of LDHB in Human serum and plasma titrations

    The concentrations of LDHB were interpolated from the LDHB standard curve and corrected for sample dilution. The mean LDHB concentration was determined to be 197 ng/mL in plasma (heparin) and 173 ng/mL in serum.

  • Interpolated concentrations of LDHB in Human serum from 10 donors
    Interpolated concentrations of LDHB in Human serum from 10 donors

    Serum from 10 apparently healthy male donors was measured in triplicate for LDHB. The mean LDHB concentration was determined to be 161 ng/mL with a range of 56 – 226 ng/mL.

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