Human Lactate Dehydrogenase B / LDH-B ELISA Kit (ab183367)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 8 pg/ml
- Range: 0.312 ng/ml - 20 ng/ml
- Sample type: Cell culture supernatant, Cell Lysate, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Lactate Dehydrogenase B / LDH-B ELISA Kit
See all Lactate Dehydrogenase B/LDH-B kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% HeLa extract 5 1.9% Inter-assay Sample n Mean SD CV% HeLa extract 3 2.7% -
Sample type
Cell culture supernatant, Serum, Tissue Extracts, Cell Lysate, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
8 pg/ml -
Range
0.312 ng/ml - 20 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 105 101% - 108% Cell culture media 108 108% - 109% Hep Plasma 104 102% - 105% EDTA Plasma 110 107% - 115% Cit plasma 110 107% - 114% Extraction Buffer 98 97% - 100% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Lactate Dehydrogenase B ELISA kit (ab183367) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LDHB protein in human serum, plasma, cell culture supernatant, cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate human LDHB with 8 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.Sensitivity:
Samples diluted in Sample Diluent NS - 8 pg/mL
Samples diluted in 1X Cell Extraction Buffer PTR - 212 pg/mL -
Notes
Functional lactate dehydrogenase are homo or hetero tetramers composed of M and H protein subunits encoded by the LDH-A and LDH-B genes, respectively. The major isozymes of skeletal muscle and liver has four muscle (M) subunits; while the main isozymes for heart muscle contains four (H) subunits. The other variants contain both types of subunits. LDHB converts pyruvate to lactate under low oxygen conditions, while in the LDH-A converts lactate into pyruvate. Elevated levels of LDH indicate tissue breakdown in hemolysis. LDH can be used as a marker of myocardial infarction, peaking at days 3 and lasting through day 10. Other disorders indicated by elevated LDH include cancer, meningitis, encephalitis, acute pancreatitis, and HIV. Defects in LDHB are a cause of hereditary LDH-B deficiency; however LDH-B deficiency is usually asymptomatic.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Lactate Dehydrogenase B / LDH-B Capture Antibody 1 x 600µl 10X Human Lactate Dehydrogenase B / LDH-B Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human Lactate Dehydrogenase B / LDH-B Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Pathway
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. -
Involvement in disease
Note=Defects in LDHB result in deficiency of lactate dehydrogenase, a condition with no clear symptomatic consequences. Lactate dehydrogenase deficiency can probably be considered a non-disease. -
Sequence similarities
Belongs to the LDH/MDH superfamily. LDH family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- Epididymis secretory protein Li 281
- HEL S 281
- L lactate dehydrogenase B chain
see all -
Database links
- Entrez Gene: 3945 Human
- Omim: 150100 Human
- SwissProt: P07195 Human
- Unigene: 446149 Human
Images
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Other - Human Lactate Dehydrogenase B / LDH-B ELISA Kit (ab183367)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of LDHB standard curve diluted in 1X Cell Extraction PTRBackground-subtracted data values (mean +/- SD) are graphed.
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Example of LDHB standard curve diluted in NSBackground-subtracted data values (mean +/- SD) are graphed.
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Titration of HeLa and HepG2 extracts within the working range of the assayRaw data in duplicate measurements are plotted. Background is represented as the dotted black line.
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Titration of Human Heart and Human Liver homogenate extracts within the working range of the assayRaw data in duplicate measurements are plotted. Background is represented as the dotted black line.
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Quantitation of LDHB expression in different cell linesInterpolated values of LDHB are plotted for the indicated cell lines based on an extract load of 20 µg/mL.
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Interpolated concentrations of LDHB in Human serum and plasma titrationsThe concentrations of LDHB were interpolated from the LDHB standard curve and corrected for sample dilution. The mean LDHB concentration was determined to be 197 ng/mL in plasma (heparin) and 173 ng/mL in serum.
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Interpolated concentrations of LDHB in Human serum from 10 donorsSerum from 10 apparently healthy male donors was measured in triplicate for LDHB. The mean LDHB concentration was determined to be 161 ng/mL with a range of 56 – 226 ng/mL.
