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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Jumonji containing proteins

Human KDM7A (JHDM1D) knockout A549 cell lysate (ab258484)

Human KDM7A (JHDM1D) knockout A549 cell lysate (ab258484)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

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Overview

  • Product name

    Human KDM7A (JHDM1D) knockout A549 cell lysate
    See all JHDM1D kits
  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    A549
  • Organism

    Human
  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon5 and 7 bp deletion in exon5 and 8 bp deletion in exon5.
  • Passage number

  • Knockout validation

    Sanger Sequencing
  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab263647 - Human KDM7A knockout A549 cell lysate 1 x 100µg
    ab255554 - Human wild-type A549 cell lysate 1 x 100µg
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Jumonji containing proteins
  • Cell type

    epithelial
  • Disease

    Carcinoma
  • STR Analysis

    Amelogenin X,Y D5S818: 11 D13S317: 11 D7S820: 8, 11 D16S539: 11, 12 vWA: 14 TH01: 8,9.3 TPOX: 8,11 CSF1PO: 10, 12

Target

  • Function

    Histone demethylase required for brain development. Specifically demethylates dimethylated 'Lys-9' and 'Lys-27' (H3K9me2 and H3K27me2, respectively) of histone H3 and monomethylated histone H4 'Lys-20' residue (H4K20Me1), thereby playing a central role in histone code. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. Demethylates H3K9me2 in absence of H3K4me3. Has activity toward H4K20Me1 only when nucleosome is used as a substrate and when not histone octamer is used as substrate.
  • Sequence similarities

    Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.
    Contains 1 JmjC domain.
    Contains 1 PHD-type zinc finger.
  • Domain

    The PHD-type zinc finger mediates the binding to H3K4me3. Binding to H3K4me3 prevents its access to H3K9me2.
    The linker region is a critical determinant of demethylase specificity. It prevents the active site of JmjC to reach the target H3K9me2 when the PHD-type zinc finger binds to H3K4me3, while it favors selectivity toward H3K27me2.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q6ZMT4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • JHDM1D
    • jmjc containing protein
    • JmjC domain-containing histone demethylation protein 1D
    • jumonji containing protein
    • KDM7_HUMAN
    • kiaa1718
    • Lysine-specific demethylase 7
    see all

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab263647 - Human KDM7A knockout A549 cell lysate 1 x 100µg
    ab255554 - Human wild-type A549 cell lysate 1 x 100µg
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Jumonji containing proteins
  • Cell type

    epithelial
  • Disease

    Carcinoma
  • STR Analysis

    Amelogenin X,Y D5S818: 11 D13S317: 11 D7S820: 8, 11 D16S539: 11, 12 vWA: 14 TH01: 8,9.3 TPOX: 8,11 CSF1PO: 10, 12

Images

  • Sanger Sequencing - Human KDM7A knockout A549 cell lysate (ab258484)
    Sanger Sequencing - Human KDM7A knockout A549 cell lysate (ab258484)

    Allele-1: 8 bp deletion in exon5

     

  • Sanger Sequencing - Human KDM7A knockout A549 cell lysate (ab258484)
    Sanger Sequencing - Human KDM7A knockout A549 cell lysate (ab258484)

    Allele-2: 7 bp deletion in exon5

     

  • Sanger Sequencing - Human KDM7A knockout A549 cell lysate (ab258484)
    Sanger Sequencing - Human KDM7A knockout A549 cell lysate (ab258484)

    Allele-3: 1 bp deletion in exon5

     

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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