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Human IL-9 ELISA Kit (ab242237)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 2.3 pg/ml
  • Range: 9.38 pg/ml - 600 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human IL-9 ELISA Kit
    See all IL-9 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    supernatant 5 6.3%
    Inter-assay
    Sample n Mean SD CV%
    supernatant 3 6.5%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    2.3 pg/ml

  • Range

    9.38 pg/ml - 600 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 110 96% - 119%
    Serum 100 92% - 111%
    Hep Plasma 101 99% - 105%
    EDTA Plasma 102 97% - 111%
    Cit plasma 101 95% - 109%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human IL-9 ELISA Kit (ab242237) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-9 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-9 with 2.3 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human IL-9 Capture Antibody 1 x 600µl
    10X Human IL-9 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human IL-9 Lyophilized recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 75BS 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Supports IL-2 independent and IL-4 independent growth of helper T-cells.

  • Sequence similarities

    Belongs to the IL-7/IL-9 family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • Cytokine P40
    • Homolog of mouse T cell and mast cell growth factor 40
    • HP40
    • IL 9
    • IL-9
    • Il9
    • IL9_HUMAN
    • Interleukin 9
    • Interleukin-9
    • Mast cell growth factor
    • MCGF
    • Megakaryoblast growth factor
    • P40
    • p40 cytokine
    • p40 T cell and mast cell growth factor
    • T cell growth factor 3
    • T cell growth factor p40
    • T-cell growth factor P40
    • TCGF 3
    see all

  • Database links

    Images

    • Other - Human IL-9 ELISA Kit (ab242237)
      Other - Human IL-9 ELISA Kit (ab242237)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Human IL-9 Standard Curve Comparison.
      Human IL-9 Standard Curve Comparison.
      Standard Curve comparison between human IL-9 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
    • Example of human IL-9 standard curve in Sample Diluent 75BS.
      Example of human IL-9 standard curve in Sample Diluent 75BS.
      The IL-9 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
    • Interpolated concentrations of native IL-9 in human HDLM-2 cell culture supernatant samples.
      Interpolated concentrations of native IL-9 in human HDLM-2 cell culture supernatant samples.
      The concentrations of IL-9 were measured in duplicates, interpolated from the IL-9 standard curves and corrected for sample dilution. Undiluted samples are as follows: HDLM-2 supernatant 1: 100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-9 concentration was determined to be 65.7 ng/ml in HDLM-2 supernatant.
    • Sandwich ELISA - Human IL-9 ELISA Kit (ab242237)
      Sandwich ELISA - Human IL-9 ELISA Kit (ab242237)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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