Human IL-6 ELISA Kit (ab178013)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.6 pg/ml
- Range: 7.8 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-6 ELISA Kit
See all IL-6 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Cell Extract 5 2.1% Inter-assay Sample n Mean SD CV% Cell Extract 3 2.4% -
Sample type
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.6 pg/ml -
Range
7.8 pg/ml - 500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 82 77% - 84% Cell culture media 100 97% - 103% EDTA Plasma 82 80% - 85% Cit plasma 84 81% - 85% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-6 ELISA kit (ab178013) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-6 protein in human serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate human IL-6 with 1.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.ASSAY SPECIFICITY This kit recognizes both native and recombinant Human IL-6 protein in serum, plasma, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit.
CROSS REACTIVITY Recombinant Human IL-6Ra was prepared at 50 ng/mL and 500 pg/mL and assayed for cross reactivity. No cross-reactivity was observed.
INTERFERENCE Recombinant Human IL-6Ra was prepared at 50 ng/mL and 500 pg/mL and tested for interference. No interference with was observed. 18.Species Reactivity This kit recognizes Human IL-6 protein. Recombinant mouse IL-6 was prepared at 500 pg/mL and assayed for cross reactivity. No cross-reactivity was observed. Please contact our Technical Support team for more information
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Notes
Interleukin 6 (IL6) is a cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells. IL6 is involved in lymphocyte and monocyte differentiation and IL-6 induces myeloma and plasmacytoma growth as well as nerve cells differentiation. B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS are all responsive to IL6. IL6 is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IL-6 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human IL-6 Capture Antibody (Lyophilized) 1 vial Human IL-6 Lyophilized Recombinant Protein (ab9627) 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. -
Involvement in disease
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Interleukin BSF 2
- B cell differentiation factor
- B cell stimulatory factor 2
see all -
Database links
- Entrez Gene: 3569 Human
- Omim: 147620 Human
- SwissProt: P05231 Human
- Unigene: 654458 Human
Images
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Other - Human IL-6 ELISA Kit (ab178013)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human IL6 standard curve comparison dataStandard curve comparison between the previous Human IL6 SimpleStep ELISA® kit and the re-developed Human IL6 SimpleStep ELISA® kit. The re-developed kit shows increased sensitivity.
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Example of human IL6 standard curveBackground-subtracted data values (mean +/- SD) are graphed.
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Example of raw data value for of human IL-6 standard curveRaw data values are shown in the table.
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Interpolated concentrations of spiked IL6 in human serum and plasma samplesThe concentrations of IL6 were measured in duplicates, interpolated from the IL6 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, and plasma (citrate) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native IL6 in human peripheral blood monocyte (PBMC) cell culture supernatant samplesThe concentrations of IL6 were measured in duplicates, interpolated from the IL6 standard curves and corrected for sample dilution. Undiluted samples are as follows: Stimulated PBMC Cell culture supernatant 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL6 concentration was determined to be 60,443 pg/mL in stimulated PBMC cell culture supernatant, and undetectable in unstimulated and media controls.
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Linearity of dilution for spiked recombinant IL-6Recombinant IL-6 was spiked into biological samples and diluted in a 2-fold dilution series in Sample Diluent NS. NL= Non linear 50% pooled serum and plasma (EDTA, Citrate) samples from healthy donors was measured in duplicate. All values were below the detectable range of the assay.
50% serum from ten individual healthy human male and female donors was measured in duplicate. All values were below the detectable range of the assay.
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Linearity of dilution for native IL-6Native IL-6 was measured in biological samples in a 2- fold dilution series. Sample dilutions are made in Sample Diluent NS.
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Sandwich ELISA - Human IL-6 ELISA Kit (ab178013)Human IL-6 concentration was interpolated from the IL-6 standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human IL-6 ELISA kit (ab178013). Wild-type and IL-6 knockout A549 cells (ab273751) were assessed in duplicate (n=2). Cells were either treated with 20 ng/mL active recombinant human IL-1 beta protein (ab259387) for 24 h to induce expression of IL-6 or not treated with IL-1 beta. Data are represented as the mean and error bars represent standard deviation.
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Sandwich ELISA - Human IL-6 ELISA Kit (ab178013)To learn more about the advantages of recombinant antibodies see here.
