Human CD229 ELISA Kit (ab277431)
Key features and details
- Sensitivity: 80 pg/ml
- Range: 81.92 pg/ml - 20000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CD229 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
80 pg/ml -
Range
81.92 pg/ml - 20000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 117.1 90% - 142% Plasma 121 90% - 145% Cell culture media 119.8 95% - 144% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human CD229 ELISA Kit (ab277431) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD229 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human CD229 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human CD229 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human CD229 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human CD229 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated Anti-Human CD229 Antibody 2 vials Anti-Human CD229 coated Microplate 1 unit Human CD229 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
May participate in adhesion reactions between T lymphocytes and accessory cells by homophilic interaction. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD229
- Cell surface molecule Ly-9
- Cell surface molecule Ly9
see all -
Database links
- Entrez Gene: 4063 Human
- Omim: 600684 Human
- SwissProt: Q9HBG7 Human
- Unigene: 403857 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.