Human IL-5RA ELISA Kit (ab155435)
Key features and details
- Sensitivity: 0.15 ng/ml
- Range: 0.164 ng/ml - 40 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human IL-5RA ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
Range
0.164 ng/ml - 40 ng/mlRecovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 114 95% - 122% Serum 86.65 70% - 97% Plasma 78.97 69% - 86% Assay duration
Multiple steps standard assaySpecies reactivity
Reacts with: HumanProduct overview
Abcam’s IL-5RA Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human IL-5RA in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human IL-5RA coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-5RA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-5RA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-5RA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Notes
Optimization may be required with urine samples.
Platform
MicroplateProperties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent C 1 x 30ml Biotinylated anti-Human IL-5 R alpha 2 vials IL-5 R alpha Microplate (12 x 8 wells) 1 unit Recombinant Human IL-5 R alpha Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Alternative names
- IL5RA
-
Database links
- SwissProt: Q01344 Human
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent C 1 x 30ml Biotinylated anti-Human IL-5 R alpha 2 vials IL-5 R alpha Microplate (12 x 8 wells) 1 unit Recombinant Human IL-5 R alpha Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Alternative names
- IL5RA
-
Database links
- SwissProt: Q01344 Human
Images
-
Typical Standard Curve
Representative standard curve using ab155435 - Assay Diluent B.
-
Typical Standard CurveRepresentative standard curve using ab155435 - Assay Diluent C.