Human IL-23 ELISA Kit (ab221837)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 15.6 pg/ml
- Range: 39.1 pg/ml - 2500 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-23 ELISA Kit
See all IL-23 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 4% Inter-assay Sample n Mean SD CV% Serum 3 2.7% -
Sample type
Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
15.6 pg/ml -
Range
39.1 pg/ml - 2500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 113 108% - 120% Cell culture media 118 114% - 124% Hep Plasma 83 80% - 86% EDTA Plasma 113 110% - 116% Cit plasma 107 103% - 110% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-23 ELISA Kit (ab221837) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-23 protein in cell culture media, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-23 with 15.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
IL-23 acts as a growth factor for activated T and NK cells. IL-23 induces auto immune inflammation and may be responsible for auto immune inflammatory diseases. IL-23 is a complexed heterodimer of the IL-23A (19 subunit) and IL-12B (p40 subunit). The ab221837 pair recognize the heterodimer by using antibodies to both the p19 and p40 subunits.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IL-23 Capture Antibody 1 x 600µl 10X Human IL-23 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human IL-23 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. -
Tissue specificity
Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level). -
Sequence similarities
Belongs to the IL-6 superfamily. -
Developmental stage
Expressed by newborns dendritic cells. -
Cellular localization
Secreted. Secreted upon association with IL12B. - Information by UniProt
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Alternative names
- IL 23
- IL 23 A
- IL 23 subunit alpha
see all -
Database links
- Entrez Gene: 51561 Human
- Entrez Gene: 3593 Human
- Omim: 605580 Human
- Omim: 161561 Human
- SwissProt: Q9NPF7 Human
- SwissProt: P29460 Human
- Unigene: 382212 Human
- Unigene: 98309 Human
Images
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Other - Human IL-23 ELISA Kit (ab221837)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human IL-23 standard curveBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked IL-23 in human serum and plasma samplesThe concentrations of IL-23 were measured in duplicates, interpolated from the IL-23 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50% and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native IL-23 in human cell culture supernatant samplesThe concentrations of IL-23 were measured in duplicates, interpolated from the IL-23 standard curves and corrected for sample dilution. Undiluted samples are as follows: DMSO and LPS stimulated THP-1 cell culture supernatant 100% and IFN-γ and LPS stimulated THP-1 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-23 concentration was determined to be 346 pg/mL in DMSO and LPS stimulated THP-1 cell culture supernatant and 834 pg/mL in IFN-γ and LPS stimulated THP-1 cell culture supernatant. Media and unstimulated THP-1 cell culture supernatant were undetectable.
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Sandwich ELISA - Human IL-23 ELISA Kit (ab221837)To learn more about the advantages of recombinant antibodies see here.
