Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human IL-17D ELISA Kit (ab277723)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 11 pg/ml
  • Range: 46.88 pg/ml - 3000 pg/ml
  • Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human IL-17D ELISA Kit
    See all IL-17D kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Plasma 8 4.1%
    Inter-assay
    Sample n Mean SD CV%
    Plasma 3 5%

  • Sample type

    Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    11 pg/ml

  • Range

    46.88 pg/ml - 3000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 85 83% - 87%
    Cell culture media 103 101% - 107%
    Hep Plasma 92 89% - 95%
    EDTA Plasma 85 83% - 88%
    Cit plasma 99 96% - 106%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human IL-17D ELISA kit (ab277723) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human IL-17D protein in human serum, plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-17D with 11 pg/mL sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            -Single-wash protocol reduces assay time to 90 minutes or less
            -High sensitivity, specificity and reproducibility from superior antibodies
            -Fully validated in biological samples
            -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    IL-17D is a secreted cytokine of IL-17 protein family. IL-17D induces expression of IL6, CXCL8/IL-8, and CSF2/GM-CSF from endothelial cells.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human IL-17D Capture Antibody 1 x 600µl
    10X Human IL-17D Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human IL-17D Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 50BP 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Induces expression of IL-6, IL-8, and GM-CSF from endothelial cells.

  • Tissue specificity

    Expressed preferentially in adipose, skeletal muscle and CNS.

  • Sequence similarities

    Belongs to the IL-17 family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • IL 17D
    • IL 22
    • IL 27
    • IL-17D
    • IL-27
    • IL17D
    • IL17D_HUMAN
    • IL27
    • Interleukin 17D
    • Interleukin 27
    • Interleukin-17D
    • Interleukin-27
    see all

  • Database links

    Images

    • SimpleStep ELISA Protocol Diagram
      SimpleStep ELISA Protocol Diagram

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

    • Example of human IL-17D standard curve in Sample Diluent 50BP.
      Example of human IL-17D standard curve in Sample Diluent 50BP.

      The IL-17D standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of spike IL-17D in human serum, plasma and cell culture supernatant samples.
      Interpolated concentrations of spike IL-17D in human serum, plasma and cell culture supernatant samples.

      The concentrations of IL-17D were measured in duplicates, interpolated from the IL-17D standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, plasma (EDTA) 100%, plasma (heparin) 100% and cell culture media (RPMI 1640 containing 10% fetal bovine serum) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

    • Recombinant Antibody Benefits
      Recombinant Antibody Benefits

      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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