Human ICAM1 ELISA Set (without plates) (ab47349)
Overview
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Product name
Human ICAM1 ELISA Set (without plates) -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
Range
0.25 ng/ml - 8 ng/mlAssay duration
Multiple steps standard assaySpecies reactivity
Reacts with: HumanProduct overview
Abcam’s ICAM1 Human in vitro ELISA Set is designed for the quantitative measurement of ICAM1 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
This ELISA set is intended to measure human ICAM1 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
The ICAM1 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.Recognizes both natural and recombinant human ICAM1.
Tested applications
Suitable for: Sandwich ELISAmore detailsPlatform
ReagentsProperties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 5 x 96 tests 10 x 96 tests 15 x 96 tests Detection biotinylated anti-ICAM1 antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml ICAM1 Capture Antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml ICAM1 Standard 5 vials 10 vials 15 vials Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials Streptavidin-HRP 1 vial 2 vials 3 vials -
Research areas
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Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. -
Sequence similarities
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsMonoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- BB2
- CD54
- ICAM-1
see all -
Database links
- Entrez Gene: 3383 Human
- SwissProt: P05362 Human
- Unigene: 643447 Human
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 5 x 96 tests 10 x 96 tests 15 x 96 tests Detection biotinylated anti-ICAM1 antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml ICAM1 Capture Antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml ICAM1 Standard 5 vials 10 vials 15 vials Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials Streptavidin-HRP 1 vial 2 vials 3 vials -
Research areas
-
Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. -
Sequence similarities
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsMonoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. -
Cellular localization
Membrane. - Information by UniProt
-
Alternative names
- BB2
- CD54
- ICAM-1
see all -
Database links
- Entrez Gene: 3383 Human
- SwissProt: P05362 Human
- Unigene: 643447 Human
Images
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ICAM1 Human ELISA Set (ab47349)