Human HGFA ELISA Kit (ab267631)
Key features and details
- Sensitivity: 0.045 ng/ml
- Range: 0.041 ng/ml - 10 ng/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human HGFA ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
0.045 ng/ml -
Range
0.041 ng/ml - 10 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 137 132% - 139% Plasma 136 127% - 145% Cell culture media 134 123% - 145% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human HGFA ELISA Kit is designed for the quantitative determination of HGFA in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human HGFA coated on a 96-well plate. Standards and samples are pipetted into the wells and HGFA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human HGFA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HGFA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Human HGFA coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human HGFA Detection Antibody 2 vials Human HGFA Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Relevance
HGF activator belongs to the peptidase family S1 and activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form. HGF Activator is produced as a zymogen, and the form found in serum is largely the inactive single-chain form. It is activated to a heterodimeric form by endoproteolytic processing. -
Cellular localization
Secreted -
Alternative names
- Hepatocyte growth factor activator
- HGF activator
- HGF activator preproprotein
see all -
Database links
- Entrez Gene: 3083 Human
- Omim: 604552 Human
- SwissProt: Q04756 Human
- Unigene: 104 Human
Images
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Standard curve
Human HGFA ELISA kit (ab267631) Standard curve.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.