Human HAI-2 ELISA Kit (ab267569)
Key features and details
- Sensitivity: 3 pg/ml
- Range: 3.28 pg/ml - 800 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human HAI-2 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Cell culture media, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3 pg/ml -
Range
3.28 pg/ml - 800 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 73 68% - 84% Plasma 70 67% - 79% Cell culture media 79 69% - 91% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human HAI-2 ELISA Kit is designed for the quantitative determination of HAI-2 in cell culture supernatants, plasma and serum samples.
Δ Note: Collect plasma using EDTA and/or Citrate as anti-coagulant. Heparin is not recommended.
This assay employs an antibody specific for human HAI-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and HAI-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human HAI-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HAI-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA antibody pair detects human HAI-2, less than 0.1% cross-reactivity with human HAI-1 and mouse HAI-2B is observed.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Human HAI-2 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human HAI-2 Detection Antibody 2 vials Human HAI-2 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Inhibitor of HGF activator. Also inhibits plasmin, plasma and tissue kallikrein, and factor XIa. -
Tissue specificity
Expressed in placenta, kidney, pancreas, prostate, testis, thymus, and trachea. -
Involvement in disease
Defects in SPINT2 are the cause of diarrhea type 3 (DIAR3) [MIM:270420]; also known as congenital sodium diarrhea (CSD). DIAR3 is a rare, inherited diarrhea of infancy. A diagnosis of DIAR3 is made on the findings of a life-threatening secretory diarrhea, severe metabolic acidosis, and hyponatremia secondary to extraordinarily high fecal losses of sodium, with low or normal excretion of urinary sodium, in the absence of infectious, autoimmune, and endocrine causes. -
Sequence similarities
Contains 2 BPTI/Kunitz inhibitor domains. -
Domain
This inhibitor contains two inhibitory domains. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- AL024025
- Bikunin, placental
- C76321
see all -
Database links
- Entrez Gene: 10653 Human
- Omim: 605124 Human
- SwissProt: O43291 Human
- Unigene: 31439 Human
Images
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Standard curve
Human HAI-2 ELISA kit (ab267569) Standard curve.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.