Human GSTT2B (GSTT2) knockout HeLa cell line (ab264703)
Properties
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Number of cells
1 x 106 cells/vial, 1 mL -
Viability
~90% -
Adherent /Suspension
Adherent -
Tissue
Cervix -
Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10 -
Mycoplasma free
Yes -
Storage instructions
Shipped on Dry Ice. Store in liquid nitrogen. -
Storage buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether -
Research areas
Images
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Western blot - Human GSTT2B knockout HeLa cell line (ab264703)All lanes : Anti-GSTT2 antibody [EPR8136(2)] (ab176336) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : GSTT2B knockout HeLa cell lysate
Lane 3 : Human lung tissue lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 28 kDaLanes 1-4: Merged signal (red and green). Green - ab176336 observed at 28 kDa. Red - loading control, ab7291 observed at 50 kDa.
ab176336 Anti-GSTT2 antibody [EPR8136(2)] was shown to specifically react with GSTT2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264703 (knockout cell lysate ab258443) was used. Wild-type and GSTT2 knockout samples were subjected to SDS-PAGE. ab176336 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human GSTT2B knockout HeLa cell line (ab264703)Homozygous: 2 bp deletion in exon 2.
