Anti-ARFGAP1 antibody (ab85971)
Key features and details
- Rabbit polyclonal to ARFGAP1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ARFGAP1 antibody
See all ARFGAP1 primary antibodies -
Description
Rabbit polyclonal to ARFGAP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human ARFGAP1 aa 466-516.
Database link: NP_055385.2 -
Positive control
- HeLa whole cell lysate.
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General notes
This product was previously labelled as ARFGAP3
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab85971 was affinity purified using an epitope specific to ARFGAP1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ARFGAP1 antibody (ab85971)All lanes : Anti-ARFGAP1 antibody (ab85971) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 57 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
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Immunoprecipitation - Anti-ARFGAP1 antibody (ab85971)Detection of ARFGAP1 by Western Blot of Immunprecipitate.
ab85971 at 1µg/ml staining ARFGAP1 in HeLa whole cell lysate immunoprecipitated using ab85971 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
