Human GSTM1 ELISA Kit (ab277475)
Key features and details
- Sensitivity: 2 ng/ml
- Range: 2.048 ng/ml - 500 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human GSTM1 ELISA Kit
See all GSTM1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2 ng/ml -
Range
2.048 ng/ml - 500 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 94.36 69% - 137% Plasma 82.25 69% - 126% Cell culture media 88.87 69% - 126% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human GSTM1 ELISA Kit (ab277475) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human GSTM1 in serum (human GSTM1 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Human GSTM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human GSTM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human GSTM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human GSTM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated Anti-Human GSTM1 Antibody 2 vials Anti-Human GSTM1 coated Microplate 1 unit Human GSTM1 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. -
Tissue specificity
Liver (at protein level). -
Sequence similarities
Belongs to the GST superfamily. Mu family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- EC=2.5.1.18
- Glutathione S-transferase Mu 1
- GST class-mu 1
see all -
Database links
- Entrez Gene: 2944 Human
- Omim: 138350 Human
- SwissProt: P09488 Human
- Unigene: 301961 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.