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Epigenetics and Nuclear Signaling Transcription Other factors

Anti-THAP11 antibody (ab124204)

Price and availability

264 681 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-THAP11 antibody (ab124204)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to THAP11
  • Suitable for: WB
  • Reacts with: Mouse, Recombinant fragment
  • Isotype: IgG

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Overview

  • Product name

    Anti-THAP11 antibody
    See all THAP11 primary antibodies
  • Description

    Rabbit polyclonal to THAP11
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human THAP11 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab124207)

  • Positive control

    • This antibody gave a positive signal in THAP11 recombinant protein as well as the following tissue lysates: Mouse Brain; Mouse Hippocampus; Mouse Olfactory Bulb.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human THAP11 protein (ab169918)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Recombinant fragment
All applications
Rabbit
Cow
Dog
Human
Pig
Zebrafish
Macaque monkey
Gorilla
Chinese hamster
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).

Target

  • Function

    Transcriptional repressor that plays a central role for embryogenesis and the pluripotency of embryonic stem (ES) cells. Sequence-specific DNA-binding factor that represses gene expression in pluripotent ES cells by directly binding to key genetic loci and recruiting epigenetic modifiers.
  • Sequence similarities

    Belongs to the THAP11 family.
    Contains 1 THAP-type zinc finger.
  • Cellular localization

    Nucleus. Cytoplasm. May be regulated by shuttling of the protein between the cytoplasm and nucleus.
  • Target information above from: UniProt accession Q96EK4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 539845 Cow
    • Entrez Gene: 57215 Human
    • Entrez Gene: 59016 Mouse
    • Entrez Gene: 406390 Zebrafish
    • Omim: 609119 Human
    • SwissProt: A5PKF5 Cow
    • SwissProt: Q96EK4 Human
    • SwissProt: Q9JJD0 Mouse
    • SwissProt: Q6TGZ4 Zebrafish
    • Unigene: 632200 Human
    • Unigene: 286598 Mouse
    • Unigene: 75932 Zebrafish
    see all
  • Alternative names

    • CTG B43a antibody
    • CTG B45d antibody
    • HRIHFB2206 antibody
    • RONIN antibody
    • THA11_HUMAN antibody
    • THAP 11 antibody
    • THAP domain containing 11 antibody
    • THAP domain-containing protein 11 antibody
    • Thap11 antibody
    see all

Images

  • Western blot - Anti-THAP11 antibody (ab124204)
    Western blot - Anti-THAP11 antibody (ab124204)
    All lanes : Anti-THAP11 antibody (ab124204) at 1 µg

    Lane 1 : THAP11 Recombinant protein at 0.1 µg
    Lane 2 : Brain (Mouse) Tissue Lysate at 10 µg
    Lane 3 : Mouse Hippocampus Tissue Lysate at 10 µg
    Lane 4 : Mouse Olfactory Bulb Lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 34 kDa
    Observed band size: 39 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 130 kDa, 60 kDa, 75 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab124204? Please let us know so that we can cite the reference in this datasheet.

    ab124204 has not yet been referenced specifically in any publications.

    Images

    • Western blot - Anti-THAP11 antibody (ab124204)
      Western blot - Anti-THAP11 antibody (ab124204)
      All lanes : Anti-THAP11 antibody (ab124204) at 1 µg

      Lane 1 : THAP11 Recombinant protein at 0.1 µg
      Lane 2 : Brain (Mouse) Tissue Lysate at 10 µg
      Lane 3 : Mouse Hippocampus Tissue Lysate at 10 µg
      Lane 4 : Mouse Olfactory Bulb Lysate at 10 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 34 kDa
      Observed band size: 39 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 130 kDa, 60 kDa, 75 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 30 seconds

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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