Anti-THAP11 antibody (ab124204)
Key features and details
- Rabbit polyclonal to THAP11
- Suitable for: WB
- Reacts with: Mouse, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-THAP11 antibody
See all THAP11 primary antibodies -
Description
Rabbit polyclonal to THAP11 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Recombinant fragment
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Immunogen
Synthetic peptide corresponding to Human THAP11 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab124207) -
Positive control
- This antibody gave a positive signal in THAP11 recombinant protein as well as the following tissue lysates: Mouse Brain; Mouse Hippocampus; Mouse Olfactory Bulb.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124204 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB Recombinant fragmentAll applications RabbitCowDogHumanPigZebrafishMacaque monkeyGorillaChinese hamsterApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).Target
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Function
Transcriptional repressor that plays a central role for embryogenesis and the pluripotency of embryonic stem (ES) cells. Sequence-specific DNA-binding factor that represses gene expression in pluripotent ES cells by directly binding to key genetic loci and recruiting epigenetic modifiers. -
Sequence similarities
Belongs to the THAP11 family.
Contains 1 THAP-type zinc finger. -
Cellular localization
Nucleus. Cytoplasm. May be regulated by shuttling of the protein between the cytoplasm and nucleus. - Information by UniProt
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Database links
- Entrez Gene: 539845 Cow
- Entrez Gene: 57215 Human
- Entrez Gene: 59016 Mouse
- Entrez Gene: 406390 Zebrafish
- Omim: 609119 Human
- SwissProt: A5PKF5 Cow
- SwissProt: Q96EK4 Human
- SwissProt: Q9JJD0 Mouse
see all -
Alternative names
- CTG B43a antibody
- CTG B45d antibody
- HRIHFB2206 antibody
see all
Images
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Western blot - Anti-THAP11 antibody (ab124204)All lanes : Anti-THAP11 antibody (ab124204) at 1 µg
Lane 1 : THAP11 Recombinant protein at 0.1 µg
Lane 2 : Brain (Mouse) Tissue Lysate at 10 µg
Lane 3 : Mouse Hippocampus Tissue Lysate at 10 µg
Lane 4 : Mouse Olfactory Bulb Lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa, 60 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Datasheets and documents
References (0)
ab124204 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-THAP11 antibody (ab124204)All lanes : Anti-THAP11 antibody (ab124204) at 1 µg
Lane 1 : THAP11 Recombinant protein at 0.1 µg
Lane 2 : Brain (Mouse) Tissue Lysate at 10 µg
Lane 3 : Mouse Hippocampus Tissue Lysate at 10 µg
Lane 4 : Mouse Olfactory Bulb Lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa, 60 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
