Human GSTA1 ELISA Kit (ab245717)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 14 pg/ml
- Range: 0.39 ng/ml - 25 ng/ml
- Sample type: Cell culture extracts, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human GSTA1 ELISA Kit
See all GSTA1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% urine 8 5.2% Inter-assay Sample n Mean SD CV% urine 3 9.2% -
Sample type
Urine, Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
14 pg/ml -
Range
0.39 ng/ml - 25 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 97 89% - 104% Cell culture extracts 104 89% - 111% Tissue Extracts 94 88% - 100% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human GSTA1 ELISA Kit (ab245717) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GSTA1 protein in tissue extracts, urine, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human GSTA1 with 14 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human GSTA1 Capture Antibody 1 x 600µl 10X Human GSTA1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Human GSTA1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. -
Tissue specificity
Liver. -
Sequence similarities
Belongs to the GST superfamily. Alpha family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain. -
Domain
The C-terminal domain may form a component of the hydrophobic substrate-binding site, but in contrast appears not to be directly involved in GSH binding and is not absolutely essential for catalytic activity. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- Glutathione S alkyltransferase A1
- Glutathione S aryltransferase A1
- Glutathione S transferase 2
see all -
Database links
- Entrez Gene: 2938 Human
- Omim: 138359 Human
- SwissProt: P08263 Human
- Unigene: 446309 Human
Images
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Other - Human GSTA1 ELISA Kit (ab245717)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human GSTA1 standard curve in Sample Diluent NS.The GSTA1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Example of human GSTA1 standard curve in 1X Cell Extraction Buffer PTR.The GSTA1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native GSTA1 in human urine, liver and HepG2 cell extract samples.The concentrations of GSTA1 were measured in duplicates, interpolated from the GSTA1 standard curves and corrected for sample dilution. Undiluted samples are as follows: 25% urine, 0.125 µg/mL liver, and 100 µg/mL HepG2 cell supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GSTA1 concentration was determined to be 125 ng/mL in urine.
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Sandwich ELISA - Human GSTA1 ELISA Kit (ab245717)To learn more about the advantages of recombinant antibodies see here.
