Human Glypican 1 ELISA Kit (ab267650)
Key features and details
- Sensitivity: 0.15 ng/ml
- Range: 0.164 ng/ml - 40 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (qualitative)
- Reacts with: Human
Overview
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Product name
Human Glypican 1 ELISA Kit
See all Glypican 1/ GPC1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (qualitative) -
Sensitivity
0.15 ng/ml -
Range
0.164 ng/ml - 40 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 96.14 86% - 102% Serum 101.4 91% - 112% Plasma 95.11 85% - 102% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Glypican 1 ELISA Kit is designed for the quantitative determination of Glypican 1 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human Glypican 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Glypican 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Glypican 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Glypican 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: human ALK-1, B7-H2, BLAME, BMP-8, CD28, Common beta Chain, Contactin-1, Desmoglein-1, Desmoglein-3, EDAR, EphA1, EphB6, Ephrin-B3, Epiregulin, FGF-12, FGF17, FOLR2, Galectin-8, Glypican 5, GHR, IFN-gamma R1, IL-22 R alpha 1, IL-22BP, IL-23 R, IL-31 RA, IL-7 R alpha, Integrin alpha 5, MDM2, Nectin-1, NKp30, Nogo,Receptor, Notch-3, OSM R beta, Prolactin R, RELT, Ryk, Semaphorin 6D, Semaphorin 7A, Siglec-11.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Human Glypican 1 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human Glypican 1 Detection Antibody 2 vials Human Glypican 1 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Cell surface proteoglycan that bears heparan sulfate. -
Sequence similarities
Belongs to the glypican family. -
Post-translational
modificationsThis cell-associated glypican is further processed to give rise to a medium-released species. -
Cellular localization
Cell membrane and Secreted > extracellular space. - Information by UniProt
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Alternative names
- FLJ38078
- glypican
- Glypican proteoglycan 1
see all -
Database links
- Entrez Gene: 2817 Human
- Omim: 600395 Human
- SwissProt: P35052 Human
- Unigene: 328232 Human
Images
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Example standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.