Human Gelsolin ELISA Kit (ab270215)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 145.98 pg/ml
- Range: 546.88 pg/ml - 35000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Gelsolin ELISA Kit
See all Gelsolin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 2.2% Inter-assay Sample n Mean SD CV% Serum 3 4% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
145.98 pg/ml -
Range
546.88 pg/ml - 35000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 104 94% - 114% Hep Plasma 90 87% - 92% EDTA Plasma 94 92% - 97% Cit plasma 90 81% - 94% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Gelsolin ELISA Kit (ab270215) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Gelsolin protein in edta plasma, serum, cit plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Gelsolin with 145.98 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Gelsolin (GSN) is an 82-kD protein with six homologous subdomains, referred to as S1-S6. Each subdomain is composed of a five-stranded β-sheet, flanked by two α-helices, one positioned perpendicular with respect to the strands and one positioned parallel. The β-sheets of the three N-terminal subdomains (S1-S3) join to form an extended β-sheet, as do the β-sheets of the C-terminal subdomains (S4-S6). It is regulated by calcium and modulates actin by binding to the plus (or barbed) ends of actin filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Gelsolin plays a role in ciliogenesis. Human Gelsolin shares 99%, 93%, 93%, and 96% sequence homology with monkey, mouse, rat, and bovine, respectively.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Gelsolin Capture Antibody 1 x 600µl 10X Human Gelsolin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human Gelsolin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis. -
Tissue specificity
Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells. -
Involvement in disease
Defects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure. -
Sequence similarities
Belongs to the villin/gelsolin family.
Contains 6 gelsolin-like repeats. -
Post-translational
modificationsPhosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids. -
Cellular localization
Cytoplasm > cytoskeleton and Secreted. - Information by UniProt
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Alternative names
- Actin depolymerizing factor
- Actin-depolymerizing factor
- ADF
see all -
Database links
- Entrez Gene: 2934 Human
- Omim: 137350 Human
- SwissProt: P06396 Human
- Unigene: 522373 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Gelsolin standard curve in Sample Diluent NS.The Gelsolin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Gelsolin in human serum and plasma samples.The concentrations of Gelsolin were measured in duplicates, interpolated from the Gelsolin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:1000, plasma (citrate) 1:1000, plasma (EDTA) 1:1000, and plasma (Heparin) 1:2000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Gelsolin concentration was determined to be 12.54 µg/mL in serum, 18.80 µg/mL in plasma (citrate), 8.41 µg/mL in plasma (EDTA), and 48.98 µg/mL in plasma (Heparin).
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Sandwich ELISA - Human Gelsolin ELISA Kit (ab270215)To learn more about the advantages of recombinant antibodies see here.
