Human Gas 1 ELISA Kit (ab267608)
Key features and details
- Sensitivity: 45 pg/ml
- Range: 46.88 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Gas 1 ELISA Kit
See all Gas1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
45 pg/ml -
Range
46.88 pg/ml - 3000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 99.34 84% - 116% Plasma 91.77 76% - 106% Cell culture media 98.45 82% - 118% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Gas 1 ELISA Kit is designed for the quantitative determination of Gas 1 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human Gas 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Gas 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Gas 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Gas 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Human Gas 1 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human Gas 1 Detection Antibody 2 vials Human Gas 1 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Specific growth arrest protein involved in growth suppression. Blocks entry to S phase. Prevents cycling of normal and transformed cells. -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- GAS-1
- Gas1
- GAS1_HUMAN
see all -
Database links
- Entrez Gene: 2619 Human
- Omim: 139185 Human
- SwissProt: P54826 Human
- Unigene: 65029 Human
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.