Human GAD ELISA Kit (ab243677)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 304.3 pg/ml
- Range: 781.3 pg/ml - 50000 pg/ml
- Sample type: Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human GAD ELISA Kit
See all GAD67 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% extract 8 6% Inter-assay Sample n Mean SD CV% extract 3 2.9% -
Sample type
Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
304.3 pg/ml -
Range
781.3 pg/ml - 50000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Tissue Extracts 106 104% - 107% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human GAD ELISA Kit (ab243677) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GAD protein in tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human GAD with 304.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human GAD Capture Antibody 1 x 600µl 10X Human GAD Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Human GAD Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Catalyzes the production of GABA. -
Tissue specificity
Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. -
Involvement in disease
Defects in GAD1 are the cause of cerebral palsy spastic quadriplegic type 1 (CPSQ1) [MIM:603513]. A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture. -
Sequence similarities
Belongs to the group II decarboxylase family. - Information by UniProt
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Alternative names
- 67 kDa glutamic acid decarboxylase
- CPSQ1
- DCE1
see all -
Database links
- Entrez Gene: 2571 Human
- Omim: 605363 Human
- SwissProt: Q99259 Human
- Unigene: 420036 Human
Images
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Other - Human GAD ELISA Kit (ab243677)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human GAD standard curve in 1X Cell Extraction Buffer PTR.The GAD standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native GAD in human brain tissue extract based on a 250 µg/mL extract load.The concentrations of GAD were measured in duplicate and interpolated from the GAD standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GAD concentration was determined to be 4954 pg/mL in human brain tissue extract.
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Sandwich ELISA - Human GAD ELISA Kit (ab243677)To learn more about the advantages of recombinant antibodies see here.
