Human FABP4 ELISA Kit (ab234565)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.7 pg/ml
- Range: 4.69 pg/ml - 300 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human FABP4 ELISA Kit
See all FABP4 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 9 2.8% Inter-assay Sample n Mean SD CV% Serum 3 8.6% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.7 pg/ml -
Range
4.69 pg/ml - 300 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 107 105% - 111% Serum 105 101% - 107% Cell culture extracts 103 97% - 108% Tissue Extracts 104 98% - 108% Hep Plasma 106 104% - 108% EDTA Plasma 105 104% - 106% Cit plasma 108 107% - 110% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human FABP4 ELISA Kit (ab234565) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FABP4 protein in cell culture extracts, cell culture supernatant, serum, tissue extracts, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human FABP4 with 2.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human FABP4 Capture Antibody 1 x 600µl 10X Human FABP4 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Human FABP4 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. -
Cellular localization
Cytoplasm. Nucleus. Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export. - Information by UniProt
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Alternative names
- 3T3-L1 lipid-binding protein
- 422/aP2
- A-FABP
see all -
Database links
- Entrez Gene: 2167 Human
- Omim: 600434 Human
- SwissProt: P15090 Human
- Unigene: 391561 Human
Images
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Other - Human FABP4 ELISA Kit (ab234565)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human FABP4 Competitor Std Curve Comparison.Standard curve comparison between human FABP4 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity
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Example of human FABP4 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of human FABP4 standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native FABP4 in human serum and plasma samples.The concentrations of FABP4 were measured in duplicates, interpolated from the FABP4 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:80, plasma (heparin) 1:67, plasma (EDTA) 1:67, and plasma (citrate) 1:67. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FABP4 concentration in the neat samples was determined to be 13 ng/mL in serum, 12 ng/mL in plasma (heparin), 7 ng/mL in plasma (EDTA), and 11 ng/mL in plasma (citrate).
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Interpolated concentrations of native FABP4 in human THP-1 cell supernatant, THP-1 cell extract, adipocyte cell extract, and heart extract.The concentrations of FABP4 were measured in duplicate and interpolated from the FABP4 standard curve and corrected for sample dilution. Undiluted samples are as follows: THP-1 cell supernatant 50%, THP-1 cell extract 50 µg/mL, adipocyte cell extract 10 µg/mL, and heart extract 1,000 ng/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Serum from ten individual healthy human female and male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FABP4 concentration was determined to be 18 ng/mL with a range of 7 – 33 ng/mL in the female donors and 12 ng/mL with a range of 8 – 15 ng/mL in the male donors.
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Sandwich ELISA - Human FABP4 ELISA Kit (ab234565)To learn more about the advantages of recombinant antibodies see here.
