Human eIF4E ELISA Kit (ab214564)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 79.4 pg/ml
- Range: 109.4 pg/ml - 7000 pg/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human eIF4E ELISA Kit
See all eIF4E kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% MCF7 extract 8 3.4% Inter-assay Sample n Mean SD CV% MCF7 extract 3 10% -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
79.4 pg/ml -
Range
109.4 pg/ml - 7000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Tissue Extracts 108 106% - 111% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human eIF4E ELISA Kit (ab214564) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of eIF4E protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human eIF4E with 34.69 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Eukaryotic translation initiation factor 4E (eIF4E) plays a role in the initial stages of translation by recognizing the 7-methyl-guanosine cap on the 5’ end of mRNA. By unwinding mRNA secondary structure, it facilitates ribosomal recruitment. eIF4E is also a part of a complex containing CYFIP1 and FMR1 that can repress translation.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 10X Human eIF4E Capture Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BR 1 x 6ml Human eIF4E Detector Antibody Concentrate 1 x 20µl Human eIF4E Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. -
Sequence similarities
Belongs to the eukaryotic initiation factor 4E family. -
Post-translational
modificationsPhosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex. - Information by UniProt
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Alternative names
- AUTS19
- CBP
- eIF 4E
see all -
Database links
- Entrez Gene: 1977 Human
- Omim: 133440 Human
- SwissProt: P06730 Human
- Unigene: 249718 Human
Images
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Other - Human eIF4E ELISA Kit (ab214564)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human eIF4E standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native eIF4E in human MCF7, HL-60, and placenta based on a 50 µg/mL, 120 µg/mL, and 1,000 µg/mL extract load.The concentrations of eIF4E were measured in duplicate and interpolated from the eIF4E standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean eIF4E concentration was determined to be 4,667 pg/mL in MCF7, 2,899 pg/mL in HL‑60, and 3,021 pg/mL in placenta.
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Sandwich ELISA - Human eIF4E ELISA Kit (ab214564)To learn more about the advantages of recombinant antibodies see here.
