Human EGFR Phosphorylation Antibody Array - Membrane (17 Targets) (ab134005)
Overview
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Product name
Human EGFR Phosphorylation Antibody Array - Membrane (17 Targets) -
Sample type
Tissue Extracts, Cell Lysate -
Assay type
Semi-quantitative -
Species reactivity
Reacts with: Human -
Product overview
ab134005 is for simultaneous detection of the relative levels of 17 different Phosphorylated Human EGF Receptors. Suitable for cell and tissue lysates only.
Targets: EGFR (Tyr845), EGFR (Tyr992), EGFR (Tyr1045), EGFR (Tyr1068), EGFR (Tyr1086), EGFR (Tyr1148), EGFR (Tyr1173), EGFR (Ser1046/1047), EGFR (Ser1070), ErbB2 (Tyr877), ErbB2 (Tyr1112), ErbB2 (Tyr 1221/1222), ErbB2 (Tyr1248), ErbB2 (Thr686), ErbB2 (Ser1113), ErbB3 (Tyr1289), ErbB4 (Tyr1284)
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: Multiplex Protein Detectionmore details
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 4 Membranes 1 x 8 Membranes 1 x 2 Membranes 1,000X HRP-Conjugated Streptavidin 1 x 18µl 1 x 18µl 1 x 18µl 100X Phosphatase Inhibitor Cocktail Set I Concentrate 1 vial 2 vials 1 vial 1X Blocking Buffer 1 x 25ml 2 x 25ml 1 x 25ml 20X Wash Buffer I 1 x 30ml 1 x 30ml 1 x 30ml 20X Wash Buffer II 1 x 30ml 1 x 30ml 1 x 30ml 2X Cell Lysis Buffer 1 x 5ml 1 x 5ml 1 x 5ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit Cocktail of Biotin-Conjugated Anti-EGFR 2 vials 4 vials 1 vial Detection Buffer C 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml EGFR Phosphorylation Antibody Array Membranes 4 units 8 units 2 units Phosphatase Inhibitor Cocktail Set II 1 vial 2 vials 1 vial Protease Inhibitor Cocktail 1 vial 2 vials 1 vial -
Research areas
Images
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Multiplex Protein Detection - Human EGFR Phosphorylation Antibody Array - Membrane (17 Targets) (ab134005)
Human epidermoid carcinoma cell line, A431 cells that were 80-90% confluent were serum starved overnight, then exposed to 100 ng/ml EGF for 20 minutes at 37°C. Control cells were serum starved without the subsequent stimulation with EGF. Cell lysates were prepared according to the protocol. To use ab134005 Human EGFR Phosphorylation Antibody Array - Membrane, treated or untreated cell lysate was added into antibody array membrane. The antibody array membranes were washed and cocktail of biotinylated anti-EGFR was used to detect phosphorylated proteins on activated receptors. After incubation with HRP-Conjugated Streptavidin, the signals were visualized by chemiluminescence.
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Western blot - Human EGFR Phosphorylation Antibody Array - Membrane (17 Targets) (ab134005)Western blot analysis of extracts from 100 ng/ml hEGF treated A431 cells or untreated A431 cells. Phospho-EGFR (Tyr845) or Phospho-EGFR (Tyr1173) antibodies were used in this assay.
