Human DDX58 (RIG-I) knockout A549 cell lysate (ab257916)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab262254 - Human DDX58 knockout A549 cell lysate 1 x 100µg ab255554 - Human wild-type A549 cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Carcinoma -
STR Analysis
Amelogenin X,Y D5S818: 11 D13S317: 11 D7S820: 8, 11 D16S539: 11, 12 vWA: 14 TH01: 8,9.3 TPOX: 8,11 CSF1PO: 10, 12
Images
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Western blot - Human DDX58 (RIG-I) knockout A549 cell lysate (ab257916)
Lane 1: Wild-type A549 cell lysate 20 ug
Lane 2: DDX58 knockout A549 cell lysate 20 ug
Lanes 1 - 2:Merged signal (red and green). Green - ab180675 observed at 107 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab180675 was shown to react with DDX58 in wild-type A549 cells in western blot with loss of signal observed in DDX58 knockout cell line ab267116 (DDX58 knockout cell lysate ab257916). Wild-type and DDX58 knockout A549 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab180675 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively.. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human DDX58 knockout A549 cell lysate (ab257916)Allele-1: 5 bp deletion in exon2
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Sanger Sequencing - Human DDX58 knockout A549 cell lysate (ab257916)Allele-2: 2 bp deletion in exon2