Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 90 460 ₸

Order now and get it on Tuesday March 09, 2021

Anti-GSDMC antibody [EPR20887-50] (ab225635)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20887-50] to GSDMC
Suitable for: WB
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-GSDMC antibody [EPR20887-50]
See all GSDMC primary antibodies
Description

Rabbit monoclonal [EPR20887-50] to GSDMC
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Mouse

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse stomach and esophagus tissue lysates; HEK-293T transfected with mGSDMC1 expression vector, whole cell lysate.
General notes
The gene for mouse GSDMC is also known as GSDMC1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20887-50
Isotype

IgG
Research areas

Western blot - Anti-GSDMC antibody [EPR20887-50] (ab225635)

All lanes : Anti-GSDMC antibody [EPR20887-50] (ab225635) at 1/1000 dilution

Lane 1 : Mouse stomach tissue lysate
Lane 2 : Mouse esophagus tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 53 kDa
Observed band size: 52 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure times: Lane 1: 15 seconds Lane 2: 3.25 seconds.
Western blot - Anti-GSDMC antibody [EPR20887-50] (ab225635)

All lanes : Anti-GSDMC antibody [EPR20887-50] (ab225635) at 1/1000 dilution

Lane 1 : Wild-type Mouse esophagus tissue lysate
Lane 2 : mGSDMC1-4 knockout mouse esophagus tissue lysate
Lane 3 : Wild-type Mouse stomach tissue lysate
Lane 4 : mGSDMC1-4 knockout mouse stomach tissue lysate
Lane 5 : Wild-type Mouse small intestine tissue lysate
Lane 6 : mGSDMC1-4 knockout mouse small intestine tissue lysate
Lane 7 : Wild-type Mouse colon tissue lysate
Lane 8 : mGSDMC1-4 knockout mouse colon tissue lysate
Lane 9 : Wild-type Mouse caecum tissue lysate
Lane 10 : mGSDMC1-4 knockout mouse caecum tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 53 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The image was kindly provided by Dr. Jiahuai Han (Xiamen University, China).

Exposure time: 60 seconds.
Western blot - Anti-GSDMC antibody [EPR20887-50] (ab225635)

All lanes : Anti-GSDMC antibody [EPR20887-50] (ab225635) at 1/1000 dilution

Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), whole cell lysate
Lane 2 : HEK-293T transfected with mGSDMC1 expression vector, whole cell lysate
Lane 3 : HEK-293T transfected with mGSDMC2 expression vector, whole cell lysate
Lane 4 : HEK-293T transfected with mGSDMC3 expression vector, whole cell lysate
Lane 5 : HEK-293T transfected with mGSDMC4 expression vector, whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 53 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The image was kindly provided by Dr. Jiahuai Han (Xiamen University, China).

Exposure time: 60 seconds.
Anti-GSDMC antibody [EPR20887-50] (ab225635)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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