Human Cytokeratin 8 ELISA Kit (ab267810)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.01 ng/ml
- Range: 1.25 ng/ml - 80 ng/ml
- Sample type: Cell culture media, Cell Lysate, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Cytokeratin 8 ELISA Kit
See all Cytokeratin 8 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 3.7% -
Sample type
Tissue Extracts, Cell Lysate, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
1.01 ng/ml -
Range
1.25 ng/ml - 80 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Tissue Extracts 102 % - % Cell Lysate 101 % - % Cell culture media 110 % - % -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Cytokeratin 8 ELISA Kit (ab267810) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cytokeratin 8 protein in cell culture media, tissue extracts, and cell lysate. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cytokeratin 8 with 1.01 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Keratin, type II cytoskeletal 8 is a major component of the intermediate filament cytoskeleton and is a marker for epithelial cells. Abnormal expression of Cytokeratin 8 is found in various forms of cancer, and Cytokeratin 8 has been used as a marker for circulating tumor cells.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Cytokeratin 8 Capture Antibody 1 x 600µl 10X Human Cytokeratin 8 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml Human Cytokeratin 8 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
Tissue specificity
Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. -
Involvement in disease
Cirrhosis -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsPhosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. -
Cellular localization
Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix. - Information by UniProt
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Alternative names
- CARD2
- CK 8
- CK-8
see all -
Database links
- Entrez Gene: 3856 Human
- Omim: 148060 Human
- SwissProt: P05787 Human
- Unigene: 533782 Human
- Unigene: 708445 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Cytokeratin 8 standard curve in 1X Cell Extraction Buffer PTR.The Cytokeratin 8 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Cytokeratin 8 in human MCF7, HepG2, and liver homogenate extract samples based on 6.25 µg/mL, 31.25 µg/mL, and 100 µg/mL extract loads, respectively.The concentrations of Cytokeratin 8 were measured in duplicate and interpolated from the Cytokeratin 8 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytokeratin 8 concentration was determined to be 26.10 ng/mL in MCF7 extract, 10.03 ng/ml in HepG2 extract, and 6.71 ng/ml in liver homogenate extract.
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Sandwich ELISA - Human Cytokeratin 8 ELISA Kit (ab267810)To learn more about the advantages of recombinant antibodies see here.
